Relationship between salivary histatin 5 levels and Candida CFU counts in healthy elderly.

Jun Sugimoto, Takashi Kanehira, Hiroyuki Mizugai, Itsuo Chiba, Manabu Morita

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

OBJECTIVES: Few epidemiological studies have confirmed the antifungal activity of histatin 5 in saliva against Candida species. The purpose of this study was to examine the relationship between concentrations of histatin 5 and the number of cultivable Candida in saliva samples from elderly. METHODS: Whole saliva samples were obtained from 124 elderly people, 65 years or older, living in a rural community. The concentrations of histatin 5 in saliva samples were determined by the enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Total colony-forming units (CFUs) were counted on a selective medium for Candida. Multiple linear regression analysis was performed to determine the independent contribution of explanatory variables to Candida CFUs using age, sex, histatin 5 concentration and type of denture prosthesis as independent variables. RESULTS: Saliva samples from 104 subjects (84%) were candidal colony-positive. The youngest group (65-69 years old) showed significantly smaller Candida CFU counts than those in the older group. The mean Candida CFU count of denture wearers was significantly higher than that of non-denture wearers. Significantly negative associations were found between Candida CFU counts and histatin 5 level in the oldest group (p <0.05) and in the full-denture wearers (p <0.01). Multiple linear regression analysis revealed that Candida CFU counts were mostly associated with type of dentures, followed by histatin 5 concentration. CONCLUSION: Possible activity of histatin 5 against Candida in whole saliva of elderly people was epidemiologically confirmed. The area covered by the prostheses was a strong factor associated with the Candida CFU count.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalGerodontology
Volume23
Issue number3
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

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Histatins
Candida
Stem Cells
Saliva
Dentures
Prostheses and Implants
Linear Models
Regression Analysis
Rural Population
Epidemiologic Studies

ASJC Scopus subject areas

  • Ageing

Cite this

Relationship between salivary histatin 5 levels and Candida CFU counts in healthy elderly. / Sugimoto, Jun; Kanehira, Takashi; Mizugai, Hiroyuki; Chiba, Itsuo; Morita, Manabu.

In: Gerodontology, Vol. 23, No. 3, 09.2006, p. 164-169.

Research output: Contribution to journalArticle

Sugimoto, Jun ; Kanehira, Takashi ; Mizugai, Hiroyuki ; Chiba, Itsuo ; Morita, Manabu. / Relationship between salivary histatin 5 levels and Candida CFU counts in healthy elderly. In: Gerodontology. 2006 ; Vol. 23, No. 3. pp. 164-169.
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abstract = "OBJECTIVES: Few epidemiological studies have confirmed the antifungal activity of histatin 5 in saliva against Candida species. The purpose of this study was to examine the relationship between concentrations of histatin 5 and the number of cultivable Candida in saliva samples from elderly. METHODS: Whole saliva samples were obtained from 124 elderly people, 65 years or older, living in a rural community. The concentrations of histatin 5 in saliva samples were determined by the enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Total colony-forming units (CFUs) were counted on a selective medium for Candida. Multiple linear regression analysis was performed to determine the independent contribution of explanatory variables to Candida CFUs using age, sex, histatin 5 concentration and type of denture prosthesis as independent variables. RESULTS: Saliva samples from 104 subjects (84{\%}) were candidal colony-positive. The youngest group (65-69 years old) showed significantly smaller Candida CFU counts than those in the older group. The mean Candida CFU count of denture wearers was significantly higher than that of non-denture wearers. Significantly negative associations were found between Candida CFU counts and histatin 5 level in the oldest group (p <0.05) and in the full-denture wearers (p <0.01). Multiple linear regression analysis revealed that Candida CFU counts were mostly associated with type of dentures, followed by histatin 5 concentration. CONCLUSION: Possible activity of histatin 5 against Candida in whole saliva of elderly people was epidemiologically confirmed. The area covered by the prostheses was a strong factor associated with the Candida CFU count.",
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