Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR

Ilnaz Hariri, Alireza Sadr, Yasushi Shimada, Syozi Nakashima, Yasunori Sumi, Junji Tagami

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The aim of this work was to investigate relationship between refractive index (n) and mineral content (MC) (vol %) of enamel and dentin using swept-source optical coherence tomography (SS-OCT) and transverse microradiography (TMR). Enamel and dentin blocks were partitioned into three regions. The middle partition of each sample was covered with a nail polish to protect the sound area during exposure to the treatment solutions. Samples were demineralized in a demineralizing solution, which was refreshed once a week, for 2 months. One window was covered with acid-resistant varnish, leaving the other window exposed; the samples were placed in a solution for remineralization. Samples then were sliced into disks with thickness of 300 μm to 400 μm and placed on metal plate in order to capture cross-sectional images of sound, demineralized and remineralized regions by OCT at 1319 nm center wavelength. The n then was calculated via formula using image analysis software. Following n measurement, these specimens were further polished for the TMR analysis. Correlation between OCT n and TMR MC was examined. A significant and highly positive correlation was found between the measured n and the actual MC at the corresponding locations (Pearson correlation coefficients (r) were 0.94 and 0.97 in enamel and 0.95 and 0.91 in dentin after de-/remineralization process, respectively p < 0.05). OCT showed a potential for quantitative analysis of the mineral loss or gain by measuring of the n in vitro. Supported by the grant from the Japanese Ministry of Education, Global Center of Excellence (GCOE) Program, "International Research Center for Molecular Science in Tooth and Bone Diseases.".

Original languageEnglish
Title of host publicationLasers in Dentistry XVIII
Volume8208
DOIs
Publication statusPublished - 2012
Externally publishedYes
EventLasers in Dentistry XVIII - San Francisco, CA, United States
Duration: Jan 22 2012Jan 22 2012

Other

OtherLasers in Dentistry XVIII
CountryUnited States
CitySan Francisco, CA
Period1/22/121/22/12

Fingerprint

Microradiography
Refractometry
enamels
Enamels
Optical tomography
Optical Coherence Tomography
Dentin
Dental Enamel
Minerals
Refractive index
tomography
minerals
refractivity
tooth diseases
demineralizing
Tooth Diseases
Acoustic waves
varnishes
Industrial Oils
Varnish

Keywords

  • Dentin
  • Enamel
  • Mineral content
  • Refractive Index
  • SS-OCT

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Hariri, I., Sadr, A., Shimada, Y., Nakashima, S., Sumi, Y., & Tagami, J. (2012). Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR. In Lasers in Dentistry XVIII (Vol. 8208). [82080M] https://doi.org/10.1117/12.908290

Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR. / Hariri, Ilnaz; Sadr, Alireza; Shimada, Yasushi; Nakashima, Syozi; Sumi, Yasunori; Tagami, Junji.

Lasers in Dentistry XVIII. Vol. 8208 2012. 82080M.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hariri, I, Sadr, A, Shimada, Y, Nakashima, S, Sumi, Y & Tagami, J 2012, Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR. in Lasers in Dentistry XVIII. vol. 8208, 82080M, Lasers in Dentistry XVIII, San Francisco, CA, United States, 1/22/12. https://doi.org/10.1117/12.908290
Hariri I, Sadr A, Shimada Y, Nakashima S, Sumi Y, Tagami J. Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR. In Lasers in Dentistry XVIII. Vol. 8208. 2012. 82080M https://doi.org/10.1117/12.908290
Hariri, Ilnaz ; Sadr, Alireza ; Shimada, Yasushi ; Nakashima, Syozi ; Sumi, Yasunori ; Tagami, Junji. / Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR. Lasers in Dentistry XVIII. Vol. 8208 2012.
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