Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

A. Noda, M. Ishizawa, K. Ohno, T. Sendo, H. Noda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalToxicology Letters
Volume31
Issue number2
DOIs
Publication statusPublished - May 1986
Externally publishedYes

Keywords

  • Escherichia coli
  • Rat liver microsomes
  • metyrapone
  • non-competitive inhibition

ASJC Scopus subject areas

  • Toxicology

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