Abstract
Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.
| Original language | English |
|---|---|
| Pages (from-to) | 131-137 |
| Number of pages | 7 |
| Journal | Toxicology Letters |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
Fingerprint
Keywords
- Escherichia coli
- metyrapone
- non-competitive inhibition
- Rat liver microsomes
ASJC Scopus subject areas
- Toxicology
Cite this
Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity. / Noda, A.; Ishizawa, M.; Ohno, K.; Sendo, Toshiaki; Noda, H.
In: Toxicology Letters, Vol. 31, No. 2, 1986, p. 131-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity
AU - Noda, A.
AU - Ishizawa, M.
AU - Ohno, K.
AU - Sendo, Toshiaki
AU - Noda, H.
PY - 1986
Y1 - 1986
N2 - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.
AB - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.
KW - Escherichia coli
KW - metyrapone
KW - non-competitive inhibition
KW - Rat liver microsomes
UR - http://www.scopus.com/inward/record.url?scp=0022500742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022500742&partnerID=8YFLogxK
U2 - 10.1016/0378-4274(86)90006-8
DO - 10.1016/0378-4274(86)90006-8
M3 - Article
C2 - 3520960
AN - SCOPUS:0022500742
VL - 31
SP - 131
EP - 137
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -