Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

A. Noda, M. Ishizawa, K. Ohno, Toshiaki Sendo, H. Noda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalToxicology Letters
Volume31
Issue number2
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

hydrazine
Metabolites
Metyrapone
Microsomes
NADP
Liver
Escherichia coli
Free Radicals
Rats

Keywords

  • Escherichia coli
  • metyrapone
  • non-competitive inhibition
  • Rat liver microsomes

ASJC Scopus subject areas

  • Toxicology

Cite this

Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity. / Noda, A.; Ishizawa, M.; Ohno, K.; Sendo, Toshiaki; Noda, H.

In: Toxicology Letters, Vol. 31, No. 2, 1986, p. 131-137.

Research output: Contribution to journalArticle

Noda, A. ; Ishizawa, M. ; Ohno, K. ; Sendo, Toshiaki ; Noda, H. / Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity. In: Toxicology Letters. 1986 ; Vol. 31, No. 2. pp. 131-137.
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AU - Noda, H.

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