TY - JOUR
T1 - Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity
AU - Noda, A.
AU - Ishizawa, M.
AU - Ohno, K.
AU - Sendo, T.
AU - Noda, H.
PY - 1986/5
Y1 - 1986/5
N2 - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.
AB - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.
KW - Escherichia coli
KW - Rat liver microsomes
KW - metyrapone
KW - non-competitive inhibition
UR - http://www.scopus.com/inward/record.url?scp=0022500742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022500742&partnerID=8YFLogxK
U2 - 10.1016/0378-4274(86)90006-8
DO - 10.1016/0378-4274(86)90006-8
M3 - Article
C2 - 3520960
AN - SCOPUS:0022500742
VL - 31
SP - 131
EP - 137
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -