Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

A. Noda; M. Ishizawa; K. Ohno; T. Sendo; H. Noda

doi:10.1016/0378-4274(86)90006-8

Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

A. Noda, M. Ishizawa, K. Ohno, T. Sendo, H. Noda

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.

Original language	English
Pages (from-to)	131-137
Number of pages	7
Journal	Toxicology Letters
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/0378-4274(86)90006-8
Publication status	Published - May 1986
Externally published	Yes

Keywords

Escherichia coli
Rat liver microsomes
metyrapone
non-competitive inhibition

ASJC Scopus subject areas

Toxicology

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10.1016/0378-4274(86)90006-8

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title = "Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity",

abstract = "Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.",

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author = "A. Noda and M. Ishizawa and K. Ohno and T. Sendo and H. Noda",

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T1 - Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

AU - Noda, A.

AU - Ishizawa, M.

AU - Ohno, K.

AU - Sendo, T.

AU - Noda, H.

PY - 1986/5

Y1 - 1986/5

N2 - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.

AB - Hydrazine (Hz) mutagenicity was observed in a test using Escherichia coli B/r strain, WP2 uvrA and was enhanced by the addition of rat liver microsomal fraction containing a generating system, while the enhanced mutagenicity was diminished by the addition of metyrapone to the microsome-free levels. On the other hand, an NADPH-dependent difference spectrum of the metabolic intermediate of Hz-complex, characterized by a maximum level of 448 nm, was also inhibited by metyrapone. The results show that the oxidative intermediates, which are diimide and its precursor, hydrazine free radical [Biochem. Biophys. Res. Commun., 133 (1986) 1086], are responsible not only for hepatotoxicity but also for the enhancement of genotoxicity or mutagenicity.

KW - Escherichia coli

KW - Rat liver microsomes

KW - metyrapone

KW - non-competitive inhibition

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Relationship between oxidative metabolites of hydrazine and hydrazine-induced mutagenicity

Abstract

Keywords

ASJC Scopus subject areas

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