Relationship between hypertension and periapical lesion: an in vitro and in vivo study

Christine Men Martins, Hajime Sasaki, Kimito Hirai, Ana Cristina Andrada, João Eduardo Gomes-Filho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.

Original languageEnglish
Pages (from-to)e78
JournalBrazilian oral research
Volume30
Issue number1
DOIs
Publication statusPublished - Oct 10 2016
Externally publishedYes

Fingerprint

Hypertension
Osteoclasts
Periapical Periodontitis
Cytokines
X-Ray Microtomography
Dental Pulp
Bone Marrow Cells
Femur
Stem Cells
Enzyme-Linked Immunosorbent Assay
Phenotype
In Vitro Techniques
Proteins
Tartrate-Resistant Acid Phosphatase

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Relationship between hypertension and periapical lesion : an in vitro and in vivo study. / Martins, Christine Men; Sasaki, Hajime; Hirai, Kimito; Andrada, Ana Cristina; Gomes-Filho, João Eduardo.

In: Brazilian oral research, Vol. 30, No. 1, 10.10.2016, p. e78.

Research output: Contribution to journalArticle

Martins, Christine Men ; Sasaki, Hajime ; Hirai, Kimito ; Andrada, Ana Cristina ; Gomes-Filho, João Eduardo. / Relationship between hypertension and periapical lesion : an in vitro and in vivo study. In: Brazilian oral research. 2016 ; Vol. 30, No. 1. pp. e78.
@article{1ef0481ec8fe475fbe2a17ee4a9858e3,
title = "Relationship between hypertension and periapical lesion: an in vitro and in vivo study",
abstract = "The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.",
author = "Martins, {Christine Men} and Hajime Sasaki and Kimito Hirai and Andrada, {Ana Cristina} and Gomes-Filho, {Jo{\~a}o Eduardo}",
year = "2016",
month = "10",
day = "10",
doi = "10.1590/1807-3107BOR-2016.vol30.0078",
language = "English",
volume = "30",
pages = "e78",
journal = "Pesquisa odontologica brasileira = Brazilian oral research",
issn = "1517-7491",
publisher = "University of Sao Paolo",
number = "1",

}

TY - JOUR

T1 - Relationship between hypertension and periapical lesion

T2 - an in vitro and in vivo study

AU - Martins, Christine Men

AU - Sasaki, Hajime

AU - Hirai, Kimito

AU - Andrada, Ana Cristina

AU - Gomes-Filho, João Eduardo

PY - 2016/10/10

Y1 - 2016/10/10

N2 - The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.

AB - The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.

UR - http://www.scopus.com/inward/record.url?scp=85021855460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021855460&partnerID=8YFLogxK

U2 - 10.1590/1807-3107BOR-2016.vol30.0078

DO - 10.1590/1807-3107BOR-2016.vol30.0078

M3 - Article

C2 - 27737351

AN - SCOPUS:85021855460

VL - 30

SP - e78

JO - Pesquisa odontologica brasileira = Brazilian oral research

JF - Pesquisa odontologica brasileira = Brazilian oral research

SN - 1517-7491

IS - 1

ER -