Relationship between chromosomal α-specific suppressors of sir4-11 and polymorphism of the HMRa-bearing fragment

Bun ichiro Ono, Kazuhiro Takasugi, Yumiko Ishino-Arao, Kazuyuki Yamate, Satoru Nakagami, Akio Yamane, Sumio Shinoda

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3 Citations (Scopus)

Abstract

In the course of studying sir4-11 which is responsible for the non-mating phenotype of asd-homothallism of Saccharomyces cerevisiae, we detected chromosomal α-specific suppressors of it. By examining the mating-type cassette constitution of two strains and the spore-clones derived from a diploid culture formed by a mass mating between these strains, we obtained the following results; (1) the HMRa-bearing HindIII-BglII restriction fragment was dimorphic, as judged by size, within each tetrad, (2) while one form was common to all tetrads, the other varied among tetrads, (3) spore-clones with the α-specific suppressor of sir4-11 had the variant forms while those without had the common form, and (4) while the two parents had the common form, each independent diploid clone had the common form plus a variant form. From these results, we conclude that the mating of cells in certain combinations induces a change of DNA structure at or near HMRa in a mating-pair specific manner and that the change makes HMRa non-derepressible or non-functional when derepressed.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalCurrent Genetics
Volume24
Issue number1-2
DOIs
Publication statusPublished - Jul 1993

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Keywords

  • Genomic rearrangement
  • Homothallism
  • Life cycle
  • Mating-type control
  • S. cerevisiae
  • sir

ASJC Scopus subject areas

  • Genetics

Cite this

Ono, B. I., Takasugi, K., Ishino-Arao, Y., Yamate, K., Nakagami, S., Yamane, A., & Shinoda, S. (1993). Relationship between chromosomal α-specific suppressors of sir4-11 and polymorphism of the HMRa-bearing fragment. Current Genetics, 24(1-2), 7-11. https://doi.org/10.1007/BF00324658