Relationship among salivary antioxidant activity, cytokines, and periodontitis: The Nagasaki Island study

Naofumi Tamaki, Fumihiko Yoshino, Makoto Fukui, Hideaki Hayashida, Ayaka Yoshida, Masayasu Kitamura, Takahiro Iwasaki, Reiko Furugen, Koji Kawasaki, Mio Nakazato, Takahiro Maeda, Susumu Kokeguchi, Tatsuo Yamamoto, Masaichi Chang Il Lee, Hiro O. Ito, Toshiyuki Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim Antioxidant activities and cytokine levels in human body fluids are considered to be strongly associated with periodontitis. The aim of this study was to elucidate the relationship between salivary antioxidant activities against superoxide or hydroxyl radical, cytokines, and periodontal conditions through a community-based cross-sectional study conducted in Goto city, Japan. Materials and Methods Saliva samples were analysed for superoxide or hydroxyl radical scavenging activities and cytokine levels from 160 participants. We demonstrated that saliva contained superoxide and hydroxyl radical scavenging activities by using electron spin resonance with a spin-trapping agent. The concentrations of eight cytokines were measured using multiplex bead assays. Results There were significant differences in salivary superoxide or hydroxyl radical scavenging activity, and the levels of Interleukin-1β, Interleukin-6, and Interleukin-8 between periodontitis classifications. Multivariate stepwise logistic regression model showed that salivary superoxide and hydroxyl radical scavenging activities were significantly associated with the classification of periodontitis. In addition, salivary superoxide scavenging activity was found to have significant association with all periodontal parameters using multiple linear regression analysis. Conclusions These findings suggest that the evaluation of salivary antioxidant activities, as assessed by electron spin resonance, are associated with periodontitis and various clinical variables in community-dwelling participants (ClinicalTrials.gov number NCT01742728).

Original languageEnglish
Pages (from-to)711-718
Number of pages8
JournalJournal of Clinical Periodontology
Volume42
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Periodontitis
Islands
Superoxides
Hydroxyl Radical
Antioxidants
Cytokines
Electron Spin Resonance Spectroscopy
Saliva
Logistic Models
Independent Living
Spin Trapping
Body Fluids
Interleukin-8
Interleukin-1
Human Body
Linear Models
Interleukin-6
Japan
Cross-Sectional Studies
Regression Analysis

Keywords

  • antioxidant effect
  • electron spin resonance
  • epidemiology
  • oxidative stress
  • reactive oxygen species
  • saliva

ASJC Scopus subject areas

  • Periodontics

Cite this

Tamaki, N., Yoshino, F., Fukui, M., Hayashida, H., Yoshida, A., Kitamura, M., ... Saito, T. (2015). Relationship among salivary antioxidant activity, cytokines, and periodontitis: The Nagasaki Island study. Journal of Clinical Periodontology, 42(8), 711-718. https://doi.org/10.1111/jcpe.12438

Relationship among salivary antioxidant activity, cytokines, and periodontitis : The Nagasaki Island study. / Tamaki, Naofumi; Yoshino, Fumihiko; Fukui, Makoto; Hayashida, Hideaki; Yoshida, Ayaka; Kitamura, Masayasu; Iwasaki, Takahiro; Furugen, Reiko; Kawasaki, Koji; Nakazato, Mio; Maeda, Takahiro; Kokeguchi, Susumu; Yamamoto, Tatsuo; Lee, Masaichi Chang Il; Ito, Hiro O.; Saito, Toshiyuki.

In: Journal of Clinical Periodontology, Vol. 42, No. 8, 01.08.2015, p. 711-718.

Research output: Contribution to journalArticle

Tamaki, N, Yoshino, F, Fukui, M, Hayashida, H, Yoshida, A, Kitamura, M, Iwasaki, T, Furugen, R, Kawasaki, K, Nakazato, M, Maeda, T, Kokeguchi, S, Yamamoto, T, Lee, MCI, Ito, HO & Saito, T 2015, 'Relationship among salivary antioxidant activity, cytokines, and periodontitis: The Nagasaki Island study', Journal of Clinical Periodontology, vol. 42, no. 8, pp. 711-718. https://doi.org/10.1111/jcpe.12438
Tamaki, Naofumi ; Yoshino, Fumihiko ; Fukui, Makoto ; Hayashida, Hideaki ; Yoshida, Ayaka ; Kitamura, Masayasu ; Iwasaki, Takahiro ; Furugen, Reiko ; Kawasaki, Koji ; Nakazato, Mio ; Maeda, Takahiro ; Kokeguchi, Susumu ; Yamamoto, Tatsuo ; Lee, Masaichi Chang Il ; Ito, Hiro O. ; Saito, Toshiyuki. / Relationship among salivary antioxidant activity, cytokines, and periodontitis : The Nagasaki Island study. In: Journal of Clinical Periodontology. 2015 ; Vol. 42, No. 8. pp. 711-718.
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AU - Tamaki, Naofumi

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AU - Yoshida, Ayaka

AU - Kitamura, Masayasu

AU - Iwasaki, Takahiro

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N2 - Aim Antioxidant activities and cytokine levels in human body fluids are considered to be strongly associated with periodontitis. The aim of this study was to elucidate the relationship between salivary antioxidant activities against superoxide or hydroxyl radical, cytokines, and periodontal conditions through a community-based cross-sectional study conducted in Goto city, Japan. Materials and Methods Saliva samples were analysed for superoxide or hydroxyl radical scavenging activities and cytokine levels from 160 participants. We demonstrated that saliva contained superoxide and hydroxyl radical scavenging activities by using electron spin resonance with a spin-trapping agent. The concentrations of eight cytokines were measured using multiplex bead assays. Results There were significant differences in salivary superoxide or hydroxyl radical scavenging activity, and the levels of Interleukin-1β, Interleukin-6, and Interleukin-8 between periodontitis classifications. Multivariate stepwise logistic regression model showed that salivary superoxide and hydroxyl radical scavenging activities were significantly associated with the classification of periodontitis. In addition, salivary superoxide scavenging activity was found to have significant association with all periodontal parameters using multiple linear regression analysis. Conclusions These findings suggest that the evaluation of salivary antioxidant activities, as assessed by electron spin resonance, are associated with periodontitis and various clinical variables in community-dwelling participants (ClinicalTrials.gov number NCT01742728).

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