Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC

Mitsuyo Okazaki, Shinichi Usui, Norio Tada, Takamitsu Nakano, Katsuyuki Nakajima

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Remnant-Like Particles (RLP) isolated by an immunoseparation method are heterogeneous in their physical and biochemical properties. The objective of this study was to examine the relation between RLP-triglyceride (RLP-TG) to RLP-cholesterol (RLP-C) ratio and particle size distribution in RLP-C profiles from patients with hyperlipoproteinemia by HPLC. RLP were isolated from serum samples from 147 subjects. RLP-C and RLP-TG were quantified by respective enzymatic methods. Particle sizes of the RLP were measured using HPLC with 4 connected TSKgel LipopropakXL columns. Based on HPLC profiles of RLP-C from individual subjects, three different types were classified: predominantly LDL, predominantly VLDL, and mostly VLDL types. All patients with type III hyperlipidemia were mostly VLDL type but with smaller particle size of VLDL (32 nm) than other subjects. Severe hypertriglyceridemic (TG>4.52 mmoll-1) subjects were mostly VLDL type with large particle size (41 nm). As for all subjects (n=105) without predominantly LDL type, a significant correlation between RLP particle size and RLP-TG to RLP-C ratio (r=0.432, P<0.001) was obtained, but not in case of serum TG to RLP-C ratio (r=0.062). It suggests that RLP-TG to RLP-C ratio might be used for discrimination of atherogenic smaller-sized lipoprotein from larger-sized TG-rich lipoprotein remnants. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)135-149
Number of pages15
JournalClinica Chimica Acta
Volume296
Issue number1-2
DOIs
Publication statusPublished - Jun 1 2000
Externally publishedYes

Keywords

  • HPLC
  • Hyperlipidemia
  • Immunoseparation method
  • Lipid composition of remnant lipoprotein
  • Particle size
  • Remnant-like lipoproteins

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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