Regulatory T cells function at the early stage of tumor progression in a mouse model of tongue squamous cell carcinoma

Kentaro Miki, Yorihisa Orita, Yuka Gion, Soshi Takao, Kyotaro Ohno, Mai Takeuchi, Toshihiro Ito, Hiroyuki Hanakawa, Tomoyasu Tachibana, Hidenori Marunaka, Takuma Makino, Akira Minoura, Akihiro Matsukawa, Kazunori Nishizaki, Tadashi Yoshino, Yasuharu Sato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The objective of this study was to observe the distribution of regulatory T cells (Tregs) in the development of tongue squamous cell carcinoma (SCC) and to determine the role of Tregs in the progression of tongue SCC. A mouse model of 4-nitroquinoline-1-oxide (4NQO)-induced-tongue SCC was established. The expression of Forkhead box P3 (Foxp3), interleukin 10, transforming growth factor-β, chemokine CC motif ligands 17, 20, and CC chemokine receptor 4 was determined using real-time quantitative polymerase chain reaction. Foxp3 expression was also analyzed using immunohistochemistry. The results were compared with those of control mice and of 4NQO-treated mice treated with a cyclooxygenase-2 (COX-2) inhibitor. Well to moderately differentiated tongue SCC was induced in all of the experimental mice. The amount of Tregs of the experimental mice was over 10 times as much as control mice at the early stage of tumor progression. COX-2 inhibitor did not prevent the progression of tongue SCC and did not reduce the total amount of Tregs. Tregs function at the early stage of the development of tongue SCC, and it may be effective to suppress Tregs at the early stage of tumor progression for the treatment and/or prevention of tongue SCC.

Original languageEnglish
Pages (from-to)1401-1410
Number of pages10
JournalCancer Immunology, Immunotherapy
Volume65
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Regulatory T-Lymphocytes
Tongue
Squamous Cell Carcinoma
Neoplasms
4-Nitroquinoline-1-oxide
Cyclooxygenase 2 Inhibitors
CCR4 Receptors
CC Chemokines
Transforming Growth Factors
Interleukin-10
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Ligands

Keywords

  • Foxp3
  • IL-10
  • Regulatory T cell
  • TGF-β
  • Tongue squamous cell carcinoma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cite this

Regulatory T cells function at the early stage of tumor progression in a mouse model of tongue squamous cell carcinoma. / Miki, Kentaro; Orita, Yorihisa; Gion, Yuka; Takao, Soshi; Ohno, Kyotaro; Takeuchi, Mai; Ito, Toshihiro; Hanakawa, Hiroyuki; Tachibana, Tomoyasu; Marunaka, Hidenori; Makino, Takuma; Minoura, Akira; Matsukawa, Akihiro; Nishizaki, Kazunori; Yoshino, Tadashi; Sato, Yasuharu.

In: Cancer Immunology, Immunotherapy, Vol. 65, No. 11, 01.11.2016, p. 1401-1410.

Research output: Contribution to journalArticle

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