TY - JOUR
T1 - Regulation of tetracycline accumulation in Bacillus subtilis bearing B. subtilis plasmid pNS1981
AU - Sumita, Y.
AU - Shishido, K.
N1 - Funding Information:
The authors gratefully acknowledge Dr. N. Koyama for valuable suggestions during the course of the experiments. This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.
PY - 1985/12
Y1 - 1985/12
N2 - Accumulation of tetracycline (Tc) into Bacillus subtilis was studied by two methods, one involving a fluorescence assay and the other an absolute determination of accumulated drug. B. subtilis GSY908 harboring B. subtilis plasmid pNS1981 and the plasmidless host strain accumulated equivalent amounts of Tc. Prior exposure of the plasmid-harboring cells to subinhibitory concentration of Tc resulted in marked decrease in accumulation of the drug, indicating that pNS1981-determined Tc resistance is inducible. Of interest was the fact that the amount of Tc accumulated by the Tc-induced plasmid-harboring cells is increased somewhat by the addition of carboxylcyanide-m-chlorophenyl hydrazone (CCCP), an uncoupling reagent. This seems to show that energy-dependent accelerated Tc efflux is involved in decreased Tc accumulation.
AB - Accumulation of tetracycline (Tc) into Bacillus subtilis was studied by two methods, one involving a fluorescence assay and the other an absolute determination of accumulated drug. B. subtilis GSY908 harboring B. subtilis plasmid pNS1981 and the plasmidless host strain accumulated equivalent amounts of Tc. Prior exposure of the plasmid-harboring cells to subinhibitory concentration of Tc resulted in marked decrease in accumulation of the drug, indicating that pNS1981-determined Tc resistance is inducible. Of interest was the fact that the amount of Tc accumulated by the Tc-induced plasmid-harboring cells is increased somewhat by the addition of carboxylcyanide-m-chlorophenyl hydrazone (CCCP), an uncoupling reagent. This seems to show that energy-dependent accelerated Tc efflux is involved in decreased Tc accumulation.
KW - Tetracycline resistance
KW - drug influx and efflux
KW - energy inhibitor
KW - fluorescence enhancement
KW - minimum inhibitory concentration
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U2 - 10.1111/j.1574-6968.1985.tb01119.x
DO - 10.1111/j.1574-6968.1985.tb01119.x
M3 - Article
AN - SCOPUS:0022401407
SN - 0378-1097
VL - 30
SP - 403
EP - 406
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -