Regulation of sulphate assimilation in Saccharomyces cerevisiae

Bun Ichiro Ono, Kazuyasu Kijima, Nobuya Ishii, Takahiro Kawato, Akio Matsuda, Andrzej Paszewski, Sumio Shinoda

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31 Citations (Scopus)

Abstract

We examined how the activity of O-acetylserine and O-acetylhomoserine sulphydrylase (OAS/OAH) SHLase of Saccharomyces cerevisiae is affected by sulphur source added to the growth medium and genetic background of the strain. In a wild-type strain, the activity was repressed if methionine, cysteine or glutathione was added to the growth medium. However, in a strain deficient of cystathionine γ-lyase, cysteine and glutathione were repressive, but methionine was not. In strains deficient of serine O-acetyltransferase (SATase), OAS/OAH SHLase activity was low regardless of sulphur source and was further lowered by cysteine and glutathione, but not by methionine. From these observations, we concluded that S-adenosylmethionine should be excluded from being the effector for regulation of OAS/OAH SHLase. Instead, we suspected that S. cerevisiae would have the same regulatory system as Escherichia coli for sulphate assimilation; i.e. cysteine inhibits SATase to lower the cellular concentration of OAS which is required for induction of the sulphate assimilation enzymes including OAS/OAH SHLase. Subsequently, we obtained data supporting this speculation.

Original languageEnglish
Pages (from-to)1153-1162
Number of pages10
JournalYeast
Volume12
Issue number11
DOIs
Publication statusPublished - Sep 15 1996

Keywords

  • Cysteine regulon
  • O-acetylserine
  • Saccharomyces cerevisiae
  • Serine O-acetyltransferase
  • Sulphate assimilation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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  • Cite this

    Ono, B. I., Kijima, K., Ishii, N., Kawato, T., Matsuda, A., Paszewski, A., & Shinoda, S. (1996). Regulation of sulphate assimilation in Saccharomyces cerevisiae. Yeast, 12(11), 1153-1162. https://doi.org/10.1002/(SICI)1097-0061(19960915)12:11<1153::AID-YEA16>3.0.CO;2-2