Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis

Mitsutomo Abe, Hiroshi Katsumata, Yoshibumi Komeda, Taku Takahashi

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

In higher plants, the outermost cell layer (L1) of the shoot apex gives rise to the epidermis of shoot organs. Our previous study demonstrated that an 8-bp motif named the L1 box functions as a cis-regulatory element for L1-specific gene expression in the shoot system of Arabidopsis. We show here that PROTODERMAL FACTOR2 (PDF2), a member of the HD-GL2 class of homeobox genes, is expressed exclusively in the L1 of shoot meristems and that recombinant PDF2 protein specifically binds to the L1 box in vitro. Although knockout mutants of PDF2 and ATML1, another L1-specific HD-GL2 class gene sharing the highest homology with PDF2, display normal shoot development, the double mutant results in severe defects in shoot epidermal cell differentiation. This suggests that PDF2 and ATML1 are functionally interchangeable and play a critical role in maintaining the identity of L1 cells, possibly by interacting with their L1 box and those of downstream target-gene promoters.

Original languageEnglish
Pages (from-to)635-643
Number of pages9
JournalDevelopment
Volume130
Issue number4
DOIs
Publication statusPublished - Feb 2003
Externally publishedYes

Fingerprint

Homeodomain Proteins
Arabidopsis
Cell Differentiation
Long Interspersed Nucleotide Elements
Meristem
Homeobox Genes
Plant Cells
Epidermis
Genes
Gene Expression
Proteins
In Vitro Techniques

Keywords

  • Arabidopsis
  • Epidermis
  • Homeodomain
  • L1
  • MERISTEM LAYER1
  • PROTODERMAL FACTOR2
  • Shoot apical meristem

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis. / Abe, Mitsutomo; Katsumata, Hiroshi; Komeda, Yoshibumi; Takahashi, Taku.

In: Development, Vol. 130, No. 4, 02.2003, p. 635-643.

Research output: Contribution to journalArticle

Abe, Mitsutomo ; Katsumata, Hiroshi ; Komeda, Yoshibumi ; Takahashi, Taku. / Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis. In: Development. 2003 ; Vol. 130, No. 4. pp. 635-643.
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