Regulation of reactive oxygen species-mediated abscisic acid signaling in guard cells and drought tolerance by glutathione

Shintaro Munemasa, Daichi Muroyama, Hiroki Nagahashi, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

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43 Citations (Scopus)

Abstract

The phytohormone abscisic acid (ABA) induces stomatal closure in response to drought stress, leading to reduction of transpirational water loss. A thiol tripeptide glutathione (GSH) is an important regulator of cellular redox homeostasis in plants. Although it has been shown that cellular redox state of guard cells controls ABA-mediated stomatal closure, roles of GSH in guard cell ABA signaling were largely unknown. Recently we demonstrated that GSH functions as a negative regulator of ABA signaling in guard cells. In this study we performed more detailed analyses to reveal how GSH regulates guard cell ABA signaling using the GSH-deficient Arabidopsis mutant cad2-1. The cad2-1 mutant exhibited reduced water loss from rosette leaves. Whole-cell current recording using patch clamp technique revealed that the cad2-1 mutation did not affect ABA regulation of S-type anion channels. We found enhanced activation of Ca2+ permeable channels by hydrogen peroxide (H2O2) in cad2-1 guard cells. The cad2-1 mutant showed enhanced H2O2-induced stomatal closure and significant increase of ROS accumulation in whole leaves in response to ABA. Our findings provide a new understanding of guard cell ABA signaling and a new strategy to improve plant drought tolerance.

Original languageEnglish
Article number472
JournalFrontiers in Plant Science
Volume4
Issue numberNOV
DOIs
Publication statusPublished - Nov 20 2013

Fingerprint

guard cells
drought tolerance
abscisic acid
glutathione
reactive oxygen species
mutants
patch-clamp technique
tripeptides
thiols
anions
plant hormones
hydrogen peroxide
leaves
homeostasis
water stress
water
Arabidopsis
mutation
calcium

Keywords

  • Abscisic acid
  • Glutathione
  • Guard cell
  • Reactive oxygen species
  • Stomata

ASJC Scopus subject areas

  • Plant Science

Cite this

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abstract = "The phytohormone abscisic acid (ABA) induces stomatal closure in response to drought stress, leading to reduction of transpirational water loss. A thiol tripeptide glutathione (GSH) is an important regulator of cellular redox homeostasis in plants. Although it has been shown that cellular redox state of guard cells controls ABA-mediated stomatal closure, roles of GSH in guard cell ABA signaling were largely unknown. Recently we demonstrated that GSH functions as a negative regulator of ABA signaling in guard cells. In this study we performed more detailed analyses to reveal how GSH regulates guard cell ABA signaling using the GSH-deficient Arabidopsis mutant cad2-1. The cad2-1 mutant exhibited reduced water loss from rosette leaves. Whole-cell current recording using patch clamp technique revealed that the cad2-1 mutation did not affect ABA regulation of S-type anion channels. We found enhanced activation of Ca2+ permeable channels by hydrogen peroxide (H2O2) in cad2-1 guard cells. The cad2-1 mutant showed enhanced H2O2-induced stomatal closure and significant increase of ROS accumulation in whole leaves in response to ABA. Our findings provide a new understanding of guard cell ABA signaling and a new strategy to improve plant drought tolerance.",
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AU - Mori, Izumi

AU - Murata, Yoshiyuki

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