Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells

Michiyo Tanikawa, Hwa Yong Lee, Kikuko Watanabe, Magdalena Majewska, Dariusz J. Skarzynski, Soo Bong Park, Dong Seok Lee, Choon Keun Park, Thomas J. Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Interleukin-1 (IL1) has been shown to be a potent stimulator of Pilrostaglandin (PG) production in bovine endometrium. The aim of the present study was to determine the cell types in the endometrium (epithelial or stromal cells) responsible for the secretion of PGE2 and PGF2α in response to IL1A, and the intracellular mechanisms of IL1A action. Cultured bovine epithelial and stromal cells were exposed to IL1A or IL1B (0.006-3.0 nM) for 24 h. IL1A and IL1B dose-dependently stimulated PGE2 and PGF2α production in the stromal cells, but not in the epithelial cells. The stimulatory effect of IL1A (0.06-3.0 nM) on PG production was greater than that of IL1B. The stimulatory actions of IL1A on PG production was augmented by supplementing arachidonic acid (AA). When the stromal cells were incubated with IL1A and inhibitors of phospholipase (PL) C or PLA2 (1 μM; anthranitic acid), only PLA2 inhibitor completely stopped the stimulatory action of IL1A on PG production. Moreover, a specific cyclooxygenase-2 (COX2) inhibitor blocked the stimulatory effect of IL1A on PG production. IL1A (0.06 nM) promoted COX2 and microsomal PGE synthase- 1 (PGES1) gene and its protein expression. The expression of COX1, PGES2, PGES3, and PGF synthase (PGFS) mRNA was not afected by IL1A in the stromal cells. The overall results indicate that 1) the target of IL1A and IL1B for stimulating both PGE2 and PGF2α production is the stromal cells, 2) IL1A is a far more potent stimulator than IL1B on PG production in stromal cells, 3) the stimulatory effiect of IL1A on PG production is mediated via the activation of PLA2 and COX2, and (4) IL1A induced PG production by increasing expressions of COX2 and PGES1 mRNAs and their proteins in bovine stromal cells.

Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalJournal of Endocrinology
Volume199
Issue number3
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Stromal Cells
Interleukin-1
Prostaglandins
Dinoprost
Cyclooxygenase 2
Dinoprostone
prostaglandin-F synthase
Epithelial Cells
Endometrium
Prostaglandins E
Messenger RNA
Cyclooxygenase 2 Inhibitors
Type C Phospholipases
Arachidonic Acid
Proteins
Acids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Tanikawa, M., Lee, H. Y., Watanabe, K., Majewska, M., Skarzynski, D. J., Park, S. B., ... Okuda, K. (2008). Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells. Journal of Endocrinology, 199(3), 425-434. https://doi.org/10.1677/JOE-08-0237

Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells. / Tanikawa, Michiyo; Lee, Hwa Yong; Watanabe, Kikuko; Majewska, Magdalena; Skarzynski, Dariusz J.; Park, Soo Bong; Lee, Dong Seok; Park, Choon Keun; Acosta, Thomas J.; Okuda, Kiyoshi.

In: Journal of Endocrinology, Vol. 199, No. 3, 12.2008, p. 425-434.

Research output: Contribution to journalArticle

Tanikawa, M, Lee, HY, Watanabe, K, Majewska, M, Skarzynski, DJ, Park, SB, Lee, DS, Park, CK, Acosta, TJ & Okuda, K 2008, 'Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells', Journal of Endocrinology, vol. 199, no. 3, pp. 425-434. https://doi.org/10.1677/JOE-08-0237
Tanikawa M, Lee HY, Watanabe K, Majewska M, Skarzynski DJ, Park SB et al. Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells. Journal of Endocrinology. 2008 Dec;199(3):425-434. https://doi.org/10.1677/JOE-08-0237
Tanikawa, Michiyo ; Lee, Hwa Yong ; Watanabe, Kikuko ; Majewska, Magdalena ; Skarzynski, Dariusz J. ; Park, Soo Bong ; Lee, Dong Seok ; Park, Choon Keun ; Acosta, Thomas J. ; Okuda, Kiyoshi. / Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells. In: Journal of Endocrinology. 2008 ; Vol. 199, No. 3. pp. 425-434.
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abstract = "Interleukin-1 (IL1) has been shown to be a potent stimulator of Pilrostaglandin (PG) production in bovine endometrium. The aim of the present study was to determine the cell types in the endometrium (epithelial or stromal cells) responsible for the secretion of PGE2 and PGF2α in response to IL1A, and the intracellular mechanisms of IL1A action. Cultured bovine epithelial and stromal cells were exposed to IL1A or IL1B (0.006-3.0 nM) for 24 h. IL1A and IL1B dose-dependently stimulated PGE2 and PGF2α production in the stromal cells, but not in the epithelial cells. The stimulatory effect of IL1A (0.06-3.0 nM) on PG production was greater than that of IL1B. The stimulatory actions of IL1A on PG production was augmented by supplementing arachidonic acid (AA). When the stromal cells were incubated with IL1A and inhibitors of phospholipase (PL) C or PLA2 (1 μM; anthranitic acid), only PLA2 inhibitor completely stopped the stimulatory action of IL1A on PG production. Moreover, a specific cyclooxygenase-2 (COX2) inhibitor blocked the stimulatory effect of IL1A on PG production. IL1A (0.06 nM) promoted COX2 and microsomal PGE synthase- 1 (PGES1) gene and its protein expression. The expression of COX1, PGES2, PGES3, and PGF synthase (PGFS) mRNA was not afected by IL1A in the stromal cells. The overall results indicate that 1) the target of IL1A and IL1B for stimulating both PGE2 and PGF2α production is the stromal cells, 2) IL1A is a far more potent stimulator than IL1B on PG production in stromal cells, 3) the stimulatory effiect of IL1A on PG production is mediated via the activation of PLA2 and COX2, and (4) IL1A induced PG production by increasing expressions of COX2 and PGES1 mRNAs and their proteins in bovine stromal cells.",
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