Regulation of polyphosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen, mycosphaerella pinodes

Kazuhiro Toyoda, Noriaki Shirashi, Hirofumi Yoshioka, Tetsuji Yamada, Yuki Ichinose, Hachiro Oku

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on polyphosphoinositide metabolism in pea plasma membranes were examined in vitro. Lipid phosphorylation in the isolated pea plasma membrane was drastically stimulated by the elicitor, but markedly inhibited by the suppressor. A similar inhibitory effect was observed by the treatment with orthovanadate or K-252a that blocked pisatin production induced by the elicitor. Neomycin, an aminoglycoside antibiotic that interacts with the polyphosphoinositide metabolism, also affected the lipid phosphorylation in vitro and blocked the elicitor-induced accumulation of pisatin in vivo. These results suggest that rapid changes of polyphosphoinositide metabolism in pea plasma membranes is one of indispensable processes during the elicitation of defense responses.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalPlant and Cell Physiology
Volume33
Issue number4
Publication statusPublished - Jun 1992

Fingerprint

Mycosphaerella pinodes
Phosphatidylinositol Phosphates
Plasma Membrane
Peas
Pathogens
Cell membranes
Metabolism
peas
Phosphorylation
plasma membrane
pisatin
pathogen
metabolism
Cell Membrane
membrane
plasma
Lipids
pathogens
lipid
phosphorylation

Keywords

  • Defense response
  • Elicitor
  • Pisum sativum L
  • Plasma membrane
  • Polyphosphositide metaboloism
  • Suppressor

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Regulation of polyphosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen, mycosphaerella pinodes. / Toyoda, Kazuhiro; Shirashi, Noriaki; Yoshioka, Hirofumi; Yamada, Tetsuji; Ichinose, Yuki; Oku, Hachiro.

In: Plant and Cell Physiology, Vol. 33, No. 4, 06.1992, p. 445-452.

Research output: Contribution to journalArticle

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