Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation

K. Iwatsuki, H. Harada, J. Z. Zhang, K. Maruyama, F. Kaneko

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied in vivo binding sites of pemphigus antibodies and the expression of pemphigus and desmosomal antigens by keratinocytes in various culture periods. Both pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antibodies mainly bound to the desmosomal areas of the lesional skin. Desmoglein and PV antigens co-localized on the cultured normal human keratinocytes in both monolayers and stratified areas. PF antigens, frequently together with involucrin and suprabasal keratins, were expressed by stratified keratinocytes. The Western blotting study demonstrated two different desmogleins with molecular sizes of 130 and 150 kD. The 130-kD desmoglein bore PV antigenic epitopes. The 150- kD band increased in volume as cultured keratinocytes stratified. PV antigen expression on the 130-kD desmoglein precedes PF antigen formation occurring in keratinocyte differentiation.

Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalDermatology
Volume189
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Pemphigus
Keratinocytes
Desmogleins
Antigens
Desmoglein 1
Antibody Binding Sites
Keratins
Epitopes
Western Blotting
Skin
Antibodies

Keywords

  • Desmoglein
  • Desmoplakin
  • Desmosome
  • Pemphigus
  • Pemphigus vulgaris antigen
  • Plakoglobin

ASJC Scopus subject areas

  • Dermatology

Cite this

Iwatsuki, K., Harada, H., Zhang, J. Z., Maruyama, K., & Kaneko, F. (1994). Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation. Dermatology, 189, 67-71. https://doi.org/10.1159/000246934

Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation. / Iwatsuki, K.; Harada, H.; Zhang, J. Z.; Maruyama, K.; Kaneko, F.

In: Dermatology, Vol. 189, 1994, p. 67-71.

Research output: Contribution to journalArticle

Iwatsuki, K, Harada, H, Zhang, JZ, Maruyama, K & Kaneko, F 1994, 'Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation', Dermatology, vol. 189, pp. 67-71. https://doi.org/10.1159/000246934
Iwatsuki, K. ; Harada, H. ; Zhang, J. Z. ; Maruyama, K. ; Kaneko, F. / Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation. In: Dermatology. 1994 ; Vol. 189. pp. 67-71.
@article{fd0e95a27c2349c7854a64493c45c6fc,
title = "Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation",
abstract = "We studied in vivo binding sites of pemphigus antibodies and the expression of pemphigus and desmosomal antigens by keratinocytes in various culture periods. Both pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antibodies mainly bound to the desmosomal areas of the lesional skin. Desmoglein and PV antigens co-localized on the cultured normal human keratinocytes in both monolayers and stratified areas. PF antigens, frequently together with involucrin and suprabasal keratins, were expressed by stratified keratinocytes. The Western blotting study demonstrated two different desmogleins with molecular sizes of 130 and 150 kD. The 130-kD desmoglein bore PV antigenic epitopes. The 150- kD band increased in volume as cultured keratinocytes stratified. PV antigen expression on the 130-kD desmoglein precedes PF antigen formation occurring in keratinocyte differentiation.",
keywords = "Desmoglein, Desmoplakin, Desmosome, Pemphigus, Pemphigus vulgaris antigen, Plakoglobin",
author = "K. Iwatsuki and H. Harada and Zhang, {J. Z.} and K. Maruyama and F. Kaneko",
year = "1994",
doi = "10.1159/000246934",
language = "English",
volume = "189",
pages = "67--71",
journal = "Dermatology",
issn = "1018-8665",
publisher = "S. Karger AG",

}

TY - JOUR

T1 - Regulation of pemphigus and desmosomal antigen expression by keratinocyte differentiation

AU - Iwatsuki, K.

AU - Harada, H.

AU - Zhang, J. Z.

AU - Maruyama, K.

AU - Kaneko, F.

PY - 1994

Y1 - 1994

N2 - We studied in vivo binding sites of pemphigus antibodies and the expression of pemphigus and desmosomal antigens by keratinocytes in various culture periods. Both pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antibodies mainly bound to the desmosomal areas of the lesional skin. Desmoglein and PV antigens co-localized on the cultured normal human keratinocytes in both monolayers and stratified areas. PF antigens, frequently together with involucrin and suprabasal keratins, were expressed by stratified keratinocytes. The Western blotting study demonstrated two different desmogleins with molecular sizes of 130 and 150 kD. The 130-kD desmoglein bore PV antigenic epitopes. The 150- kD band increased in volume as cultured keratinocytes stratified. PV antigen expression on the 130-kD desmoglein precedes PF antigen formation occurring in keratinocyte differentiation.

AB - We studied in vivo binding sites of pemphigus antibodies and the expression of pemphigus and desmosomal antigens by keratinocytes in various culture periods. Both pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antibodies mainly bound to the desmosomal areas of the lesional skin. Desmoglein and PV antigens co-localized on the cultured normal human keratinocytes in both monolayers and stratified areas. PF antigens, frequently together with involucrin and suprabasal keratins, were expressed by stratified keratinocytes. The Western blotting study demonstrated two different desmogleins with molecular sizes of 130 and 150 kD. The 130-kD desmoglein bore PV antigenic epitopes. The 150- kD band increased in volume as cultured keratinocytes stratified. PV antigen expression on the 130-kD desmoglein precedes PF antigen formation occurring in keratinocyte differentiation.

KW - Desmoglein

KW - Desmoplakin

KW - Desmosome

KW - Pemphigus

KW - Pemphigus vulgaris antigen

KW - Plakoglobin

UR - http://www.scopus.com/inward/record.url?scp=0028214544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028214544&partnerID=8YFLogxK

U2 - 10.1159/000246934

DO - 10.1159/000246934

M3 - Article

C2 - 8049568

AN - SCOPUS:0028214544

VL - 189

SP - 67

EP - 71

JO - Dermatology

JF - Dermatology

SN - 1018-8665

ER -