Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds: Successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats

Yong Tang Quan, Toshio Kukita, Yuki Ushijima, Akiko Kukita, Kengo Nagata, Ferry Sandra, Toshiyuki Watanabe, Kazuko Toh, Yutaka Okuma, Sadamichi Kawasaki, Linda Rasubala, Junpei Teramachi, Ichiko Miyamoto, Zhou Wu, Tadahiko Iijima

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Simon extracts are vitamin K1-rich food materials extracted from the leaves of the Simon sweet potato. Although vitamin K is known to stimulate bone formation, we postulated that Simon extracts also contain unknown biological compounds having the ability to regulate bone resorption. Here we prepared the vitamin K-free fraction from the Simon extracts and investigated the ability of this fraction on the differentiation of osteoclasts. A remarkable inhibitory effect of osteoclastogenesis was observed when osteoclast precursors were treated with this fraction in rat bone marrow culture systems as well as in a pure differentiation system using murine osteoclast precursor cell line. The vitamin K-free Simon extracts markedly suppressed severe bone destruction mediated by abundant osteoclasts associated with adjuvant-induced arthritis in rats. High performance liquid chromatography (HPLC) analysis revealed that the vitamin K-free Simon extracts contained three types of low molecular weight inhibitors for osteoclastogenesis; caffeic acid, chlorogenic acids and isochlorogenic acids. Among these substances, caffeic acid showed the most powerful inhibitory effects on osteoclastogenesis. Caffeic acid significantly suppressed expression of NFATc1, a key transcription factor for the induction of osteoclastogenesis. Our current study enlightened a high utility of the Simon extracts and their chemical components as effective regulators for bone resorption accompanied with inflammation and metabolic bone diseases.

Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalHistochemistry and Cell Biology
Volume125
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Keywords

  • Adjuvant-induced arthritis
  • Caffeic acid
  • Cathepsin K
  • Food materials
  • NFATc1
  • Osteoclastogenesis
  • Simon extracts

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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