Regulation of nuclear localization signal-importin α interaction by Ca2+/S100A6

Maki Takata, Seiko Shimamoto, Fuminori Yamaguchi, Masaaki Tokuda, Hiroshi Tokumitsu, Ryoji Kobayashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although the precise intracellular roles of S100 proteins are not fully understood, these proteins are thought to be involved in Ca2+-dependent diverse signal transduction pathways. In this report, we identified importin α as a novel target of S100A6. Importin α contains armadillo repeats, essential for binding to nuclear localization signals. Based on the results from GST pull-down assay, gel-shift assay, and co-immunoprecipitation, we demonstrated that S100A6 specifically interacts with the armadillo repeats of importin α in a Ca2+-dependent manner, resulting in inhibition of the nuclear localization signal (NLS)-importin α complex formation in vitro and in vivo. These results indicate S100A6 may regulate the nuclear transport of NLS-cargos in response to increasing concentrations of intracellular Ca2+. Structured summary: MINT- 8045244: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with S100A2 (uniprotkb: P29034) by pull down (MI: 0096). MINT- 8044928: Importin alpha (uniprotkb: P52292) binds (MI: 0407) to S100A6 (uniprotkb: P06703) by pull down (MI: 0096). MINT- 8044941: Importin alpha (uniprotkb: P52292) and S100A6 (uniprotkb: P06703) bind (MI: 0407) by electrophoretic mobility supershift assay (MI: 0412). MINT- 8044997: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with S100A6 (uniprotkb: P06703) by anti bait coimmunoprecipitation (MI: 0006). MINT- 8045031: Importin beta (uniprotkb: Q14974) physically interacts (MI: 0915) with importin alpha (uniprotkb: P52293) and S100A6 (uniprotkb: P06703) by pull down (MI: 0096). MINT- 8044917: Importin alpha (uniprotkb: P52292) binds (MI: 0407) to S100A2 (uniprotkb: P29034) by pull down (MI: 0096). MINT- 8045257: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with S100A6 (uniprotkb: P06703) by pull down (MI: 0096). MINT- 8045015: Importin beta (uniprotkb: Q14974) physically interacts (MI: 0915) with importin alpha (uniprotkb: P52293) and S100A2 (uniprotkb: P29034) by pull down (MI: 0096). MINT- 8045267: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with S100A2 (uniprotkb: P29034) and npm2 (uniprotkb: Q6GQG6) by pull down (MI: 0096). MINT- 8045316: Importin beta (uniprotkb: Q14974) physically interacts (MI: 0915) with importin alpha (uniprotkb: P52293) by pull down (MI: 0096). MINT- 8045302: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with NPM1 (uniprotkb: P06748) and S100A2 (uniprotkb: P29034) by pull down (MI: 0096). MINT- 8045290: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with npm2 (uniprotkb: Q6GQG6) by pull down (MI: 0096). MINT- 8044963, MINT- 8044985: Importin alpha (uniprotkb: P52292) physically interacts (MI: 0915) with S100A2 (uniprotkb: P29034) by anti bait coimmunoprecipitation (MI: 0006). MINT- 8044951: Importin alpha (uniprotkb: P52292) and S100A2 (uniprotkb: P29034) bind (MI: 0407) by electrophoretic mobility supershift assay (MI: 0412).

Original languageEnglish
Pages (from-to)4517-4523
Number of pages7
JournalFEBS Letters
Volume584
Issue number22
DOIs
Publication statusPublished - Nov 19 2010
Externally publishedYes

Keywords

  • Armadillo repeat
  • Importin α
  • Nuclear localization signal
  • S100 proteins

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Takata, M., Shimamoto, S., Yamaguchi, F., Tokuda, M., Tokumitsu, H., & Kobayashi, R. (2010). Regulation of nuclear localization signal-importin α interaction by Ca2+/S100A6. FEBS Letters, 584(22), 4517-4523. https://doi.org/10.1016/j.febslet.2010.09.052