Regulation of nitric oxide generation by up-regulated arginase I in rat spinal cord injury

Takashi Imagama, Keiki Ogino, Kei Takemoto, Yoshihiko Kato, Hideo Kataoka, Hidenori Suzuki, Zhang Ran, Heri Setiawan, Yoshihisa Fujikura, Toshihiko Taguchi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Recently, arginase is suggested to regulate nitric oxide production by competing with nitric oxide synthase for the same substrate, L-arginine, in experimental asthma. We investigated the role of arginase and its relationship to nitric oxide production after spinal cord injury. Rats were subjected to laminectomy and complete transection of their spinal cords (injury group) or laminectomy only (sham group). In the injury group, arginase I was increased in the macrophages at the transection edge, and the peak was observed 48 h after spinal cord injury. However, nitric oxide production decreased significantly in the injury group despite increased nitric oxide synthase2 mRNA expression compared with the sham group. We also demonstrated the reduction in L-arginine concentrations, which was inversely associated with changes in arginase activity. Therefore, arginase appeared to regulate nitric oxide production by consuming L-arginine. The regulation of arginase activity and L-arginine levels may improve nitroxidative stress and reduce tissue damage in spinal cord injury.

Original languageEnglish
Pages (from-to)68-75
Number of pages8
Journaljournal of clinical biochemistry and nutrition
Volume51
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • Arginase
  • L-arginine
  • Nitric oxide
  • Nitric oxide synthase
  • Spinal cord injury

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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