Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration

Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

An amputated cricket leg regenerates all missing parts with normal size and shape, indicating that regenerating blastemal cells are aware of both their position and the normal size of the leg. However, the molecular mechanisms regulating this process remain elusive. Here, we use a cricket model to show that the Dachsous/Fat (Ds/Ft) signalling pathway is essential for leg regeneration. We found that knockdown of ft or ds transcripts by regeneration-dependent RNA interference (rdRNAi) suppressed proliferation of the regenerating cells along the proximodistal (PD) axis concomitantly with remodelling of the pre-existing stump, making the regenerated legs shorter than normal. By contrast, knockdown of the expanded (ex) or Merlin (Mer) transcripts induced over-proliferation of the regenerating cells, making the regenerated legs longer. These results are consistent with those obtained using rdRNAi during intercalary regeneration induced by leg transplantation. We present a model to explain our results in which the steepness of the Ds/Ft gradient controls growth along the PD axis of the regenerating leg.

Original languageEnglish
Pages (from-to)2235-2245
Number of pages11
JournalDevelopment
Volume136
Issue number13
DOIs
Publication statusPublished - Jul 1 2009
Externally publishedYes

Fingerprint

Regeneration
Leg
Fats
Gryllidae
RNA Interference
Neurofibromin 2
Cell Proliferation
Transplantation
Growth

Keywords

  • Dachsous/Fat
  • Expanded/Merlin
  • Legs
  • Regeneration
  • Size
  • Steepness model

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Bando, T., Mito, T., Maeda, Y., Nakamura, T., Ito, F., Watanabe, T., ... Noji, S. (2009). Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration. Development, 136(13), 2235-2245. https://doi.org/10.1242/dev.035204

Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration. / Bando, Tetsuya; Mito, Taro; Maeda, Yuko; Nakamura, Taro; Ito, Fumiaki; Watanabe, Takahito; Ohuchi, Hideyo; Noji, Sumihare.

In: Development, Vol. 136, No. 13, 01.07.2009, p. 2235-2245.

Research output: Contribution to journalArticle

Bando, T, Mito, T, Maeda, Y, Nakamura, T, Ito, F, Watanabe, T, Ohuchi, H & Noji, S 2009, 'Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration', Development, vol. 136, no. 13, pp. 2235-2245. https://doi.org/10.1242/dev.035204
Bando, Tetsuya ; Mito, Taro ; Maeda, Yuko ; Nakamura, Taro ; Ito, Fumiaki ; Watanabe, Takahito ; Ohuchi, Hideyo ; Noji, Sumihare. / Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration. In: Development. 2009 ; Vol. 136, No. 13. pp. 2235-2245.
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