Abstract
The interleukin-2 receptor (IL-2R) γ chain (γc chain) is shared by IL-4R, IL-7R, IL-9R, and IL-15R and plays an important role in regulation of the immune system. However, its regulation in monocytic cell lines has not been well clarified. We examined the expression and regulation of the IL-2Rα, IL-2Rβ, γc chain, IL-4R and IL-7R mRNA in a human monoblastic leukemia cell line, THP-1. Unstimulated THP-1 cells constitutively expressed a low level of γc chain and IL-4R mRNA, Phorbol myristate acetate (PMA) induced macrophage-like differentiation and up-regulated the γc chain mRNA expression in THP-1 cells. This effect of PMA was suppressed by the protein kinase inhibitors H-7 and staurosporine. PMA did not affect the expression of the other IL-R mRNAs examined. 1 α, 25(OH)2D3 and interferon-γ also induced differentiation of THP-1 cells, but these reagents did not affect the expression of the IL-R mRNAs in THP-1 cells. These findings suggest that the expression of the γc chain mRNA is regulated by the PMA-dependent pathway and is not associated with that of the other IL-R mRNAs.
| Original language | English |
|---|---|
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Acta Medica Okayama |
| Volume | 50 |
| Issue number | 3 |
| Publication status | Published - Jun 1996 |
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Keywords
- Differentiation
- IL-2R γ chain
- Monocyte
- Phorbol ester
- Protein kinase
ASJC Scopus subject areas
- Surgery
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Regulation of Interleukin-2 Receptor γ Chain mRNA Expression in Human Monocytic Cell Line THP-I. / Yanai, Hiroyuki; Yoshino, Tadashi; Takahashi, Kiyoshi; Ninomiya, Yoshifumi; Akagi, Tadaatsu.
In: Acta Medica Okayama, Vol. 50, No. 3, 06.1996, p. 145-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of Interleukin-2 Receptor γ Chain mRNA Expression in Human Monocytic Cell Line THP-I
AU - Yanai, Hiroyuki
AU - Yoshino, Tadashi
AU - Takahashi, Kiyoshi
AU - Ninomiya, Yoshifumi
AU - Akagi, Tadaatsu
PY - 1996/6
Y1 - 1996/6
N2 - The interleukin-2 receptor (IL-2R) γ chain (γc chain) is shared by IL-4R, IL-7R, IL-9R, and IL-15R and plays an important role in regulation of the immune system. However, its regulation in monocytic cell lines has not been well clarified. We examined the expression and regulation of the IL-2Rα, IL-2Rβ, γc chain, IL-4R and IL-7R mRNA in a human monoblastic leukemia cell line, THP-1. Unstimulated THP-1 cells constitutively expressed a low level of γc chain and IL-4R mRNA, Phorbol myristate acetate (PMA) induced macrophage-like differentiation and up-regulated the γc chain mRNA expression in THP-1 cells. This effect of PMA was suppressed by the protein kinase inhibitors H-7 and staurosporine. PMA did not affect the expression of the other IL-R mRNAs examined. 1 α, 25(OH)2D3 and interferon-γ also induced differentiation of THP-1 cells, but these reagents did not affect the expression of the IL-R mRNAs in THP-1 cells. These findings suggest that the expression of the γc chain mRNA is regulated by the PMA-dependent pathway and is not associated with that of the other IL-R mRNAs.
AB - The interleukin-2 receptor (IL-2R) γ chain (γc chain) is shared by IL-4R, IL-7R, IL-9R, and IL-15R and plays an important role in regulation of the immune system. However, its regulation in monocytic cell lines has not been well clarified. We examined the expression and regulation of the IL-2Rα, IL-2Rβ, γc chain, IL-4R and IL-7R mRNA in a human monoblastic leukemia cell line, THP-1. Unstimulated THP-1 cells constitutively expressed a low level of γc chain and IL-4R mRNA, Phorbol myristate acetate (PMA) induced macrophage-like differentiation and up-regulated the γc chain mRNA expression in THP-1 cells. This effect of PMA was suppressed by the protein kinase inhibitors H-7 and staurosporine. PMA did not affect the expression of the other IL-R mRNAs examined. 1 α, 25(OH)2D3 and interferon-γ also induced differentiation of THP-1 cells, but these reagents did not affect the expression of the IL-R mRNAs in THP-1 cells. These findings suggest that the expression of the γc chain mRNA is regulated by the PMA-dependent pathway and is not associated with that of the other IL-R mRNAs.
KW - Differentiation
KW - IL-2R γ chain
KW - Monocyte
KW - Phorbol ester
KW - Protein kinase
UR - http://www.scopus.com/inward/record.url?scp=0030158398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030158398&partnerID=8YFLogxK
M3 - Article
C2 - 8805854
AN - SCOPUS:0030158398
VL - 50
SP - 145
EP - 150
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 3
ER -