Regulation of gonadotropins by urocortin 2 in gonadotropic tumor LβT2 cells

Kazunori Kageyama, Shingo Murasawa, Kanako Niioka, Fumio Otsuka, Hiroko Yagi, Makoto Daimon

Research output: Contribution to journalArticle

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Abstract

A close interaction has been shown between the hypothalamo-pituitary-gonadal axis and the hypothalamic-pituitary-adrenal axis. Urocortin 2 (Ucn2) has a very high affinity for the corticotropin-releasing factor (CRF) type 2 (CRF2) receptor. Pituitary Ucn2 regulates expression and secretion of gonadotropins in response to stress. The CRF2 receptor in the pituitary contributes to the modulation of gonadotropins. To explore the possible function of Ucn2 and the CRF2 receptor in pituitary gonadotropic tumor cells, we examined the direct regulation of gonadotropins by Ucn2 in a representative pituitary gonadotropic tumor, mouse LβT2 cells. LβT2 cells were found to express CRF1 receptor and CRF2 receptor mRNA. Ucn2 decreased CRF1 receptor mRNA levels, while it increased CRF2 receptor mRNA levels. Ucn2 directly decreased the mRNA levels of both luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in LβT2 cells. Ucn2 also decreased gonadotropin-releasing hormone receptor (GnRHR) mRNA levels. A selective CRF2 receptor antagonist suppressed the Ucn2-induced decreases in LH, FSH, and GnRHR mRNA levels. Ucn2 acts on gonadotrophs expressing the CRF2 receptor, and inhibits the production of gonadotropins in the pituitary gonadotropic tumor cells. (177 words)

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalNeuroscience Letters
Volume660
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Urocortins
Gonadotropins
LHRH Receptors
Neoplasms
Messenger RNA
Pituitary Neoplasms
Luteinizing Hormone
Gonadotrophs
Follicle Stimulating Hormone
CRF receptor type 2

Keywords

  • Corticotropin-releasing factor
  • Gonadotropin
  • Pituitary
  • Stress
  • Urocortin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulation of gonadotropins by urocortin 2 in gonadotropic tumor LβT2 cells. / Kageyama, Kazunori; Murasawa, Shingo; Niioka, Kanako; Otsuka, Fumio; Yagi, Hiroko; Daimon, Makoto.

In: Neuroscience Letters, Vol. 660, 01.11.2017, p. 63-67.

Research output: Contribution to journalArticle

Kageyama, Kazunori ; Murasawa, Shingo ; Niioka, Kanako ; Otsuka, Fumio ; Yagi, Hiroko ; Daimon, Makoto. / Regulation of gonadotropins by urocortin 2 in gonadotropic tumor LβT2 cells. In: Neuroscience Letters. 2017 ; Vol. 660. pp. 63-67.
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