TY - JOUR
T1 - Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene
AU - Mathooko, Francis M.
AU - Tsunashima, Yuki
AU - Owino, Willis Z.O.
AU - Kubo, Yasutaka
AU - Inaba, Akitsugu
N1 - Funding Information:
This research was supported by a Grant-in-Aid (no. 97127 to F.M. Mathooko) from the Japan Society for the Promotion of Science (JSPS). F.M. Mathooko was supported by a JSPS Postdoctoral Fellowship.
PY - 2001
Y1 - 2001
N2 - We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.
AB - We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.
KW - 1-Methylcyclopropene
KW - ACC oxidase
KW - ACC synthase
KW - CO
KW - Ethylene biosynthesis
KW - Peach
KW - Prunus persica L.
KW - Regulation of genes
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U2 - 10.1016/S0925-5214(00)00158-7
DO - 10.1016/S0925-5214(00)00158-7
M3 - Article
AN - SCOPUS:0035123791
SN - 0925-5214
VL - 21
SP - 265
EP - 281
JO - Postharvest Biology and Technology
JF - Postharvest Biology and Technology
IS - 3
ER -