Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene

Francis M. Mathooko, Yuki Tsunashima, Willis Z O Owino, Yasutaka Kubo, Akitsugu Inaba

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.

Original languageEnglish
Pages (from-to)265-281
Number of pages17
JournalPostharvest Biology and Technology
Volume21
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Prunus persica
1-methylcyclopropene
plant damage
Carbon Dioxide
ethylene production
peaches
ethylene
Fruit
1-aminocyclopropanecarboxylate synthase
carbon dioxide
ripening
Messenger RNA
fruits
Enzymes
Oxidoreductases
enzymes
Genes
genes
Wounds and Injuries
1-aminocyclopropane-1-carboxylate synthase

Keywords

  • 1-Methylcyclopropene
  • ACC oxidase
  • ACC synthase
  • CO
  • Ethylene biosynthesis
  • Peach
  • Prunus persica L.
  • Regulation of genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Horticulture

Cite this

Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene. / Mathooko, Francis M.; Tsunashima, Yuki; Owino, Willis Z O; Kubo, Yasutaka; Inaba, Akitsugu.

In: Postharvest Biology and Technology, Vol. 21, No. 3, 2001, p. 265-281.

Research output: Contribution to journalArticle

@article{497bee956df746f18ab55b0802840d8f,
title = "Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene",
abstract = "We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.",
keywords = "1-Methylcyclopropene, ACC oxidase, ACC synthase, CO, Ethylene biosynthesis, Peach, Prunus persica L., Regulation of genes",
author = "Mathooko, {Francis M.} and Yuki Tsunashima and Owino, {Willis Z O} and Yasutaka Kubo and Akitsugu Inaba",
year = "2001",
doi = "10.1016/S0925-5214(00)00158-7",
language = "English",
volume = "21",
pages = "265--281",
journal = "Postharvest Biology and Technology",
issn = "0925-5214",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene

AU - Mathooko, Francis M.

AU - Tsunashima, Yuki

AU - Owino, Willis Z O

AU - Kubo, Yasutaka

AU - Inaba, Akitsugu

PY - 2001

Y1 - 2001

N2 - We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.

AB - We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action.

KW - 1-Methylcyclopropene

KW - ACC oxidase

KW - ACC synthase

KW - CO

KW - Ethylene biosynthesis

KW - Peach

KW - Prunus persica L.

KW - Regulation of genes

UR - http://www.scopus.com/inward/record.url?scp=0035123791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035123791&partnerID=8YFLogxK

U2 - 10.1016/S0925-5214(00)00158-7

DO - 10.1016/S0925-5214(00)00158-7

M3 - Article

AN - SCOPUS:0035123791

VL - 21

SP - 265

EP - 281

JO - Postharvest Biology and Technology

JF - Postharvest Biology and Technology

SN - 0925-5214

IS - 3

ER -