Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells

Dirk Schenke, Kana Naito, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

INF1 elicitin, a proteinaceous elicitor produced by Phytophthora infestans, induces a hypersensitive response in tobacco BY-2 cells. In response to elicitin, tobacco cells produce both reactive oxygen species (ROS) and ethylene (ET). To investigate the regulation of elicitin-induced ET production, we pharmacologically analyzed the effects of several chemicals on ET production. Inhibitors of ROS generation or ROS chelators efficiently inhibited ET production, whereas simultaneous treatment of a superoxide anion-generating system with salicylhydroxamic acid recovered ET production. In an in vitro experiment, superoxide anion was necessary and sufficient for conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ET because ET was produced from ACC solely in the presence of the superoxide-generating chemical KO2. ET production was also inhibited by lipoxygenase (LOX) inhibitors, indicating a possible involvement of LOX-mediated generation of superoxide anion and ET production itself. Furthermore, elicitin-induced ET production was completely inhibited by the protein synthesis inhibitor cycloheximide but recovered after exogenous application of ACC, indicating that de novo protein synthesis is required for ACC accumulation, leading to ET production. We also investigated the effects of several phytohormones on elicitor-induced ET production and discuss their role in the defense response.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalJournal of General Plant Pathology
Volume71
Issue number4
DOIs
Publication statusPublished - Aug 2005

Fingerprint

ethylene production
tobacco
superoxide anion
cells
ethylene
reactive oxygen species
lipoxygenase
protein synthesis inhibitors
hypersensitive response
Phytophthora infestans
cycloheximide
chelating agents
plant hormones
protein synthesis
acids

Keywords

  • Elicitin
  • Ethylene
  • Nicotiana tabacum
  • Oxidative burst

ASJC Scopus subject areas

  • Plant Science

Cite this

Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells. / Schenke, Dirk; Naito, Kana; Toyoda, Kazuhiro; Inagaki, Yoshishige; Shiraishi, Tomonori; Ichinose, Yuki.

In: Journal of General Plant Pathology, Vol. 71, No. 4, 08.2005, p. 273-279.

Research output: Contribution to journalArticle

Schenke, Dirk ; Naito, Kana ; Toyoda, Kazuhiro ; Inagaki, Yoshishige ; Shiraishi, Tomonori ; Ichinose, Yuki. / Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells. In: Journal of General Plant Pathology. 2005 ; Vol. 71, No. 4. pp. 273-279.
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