Abstract
INF1 elicitin, a proteinaceous elicitor produced by Phytophthora infestans, induces a hypersensitive response in tobacco BY-2 cells. In response to elicitin, tobacco cells produce both reactive oxygen species (ROS) and ethylene (ET). To investigate the regulation of elicitin-induced ET production, we pharmacologically analyzed the effects of several chemicals on ET production. Inhibitors of ROS generation or ROS chelators efficiently inhibited ET production, whereas simultaneous treatment of a superoxide anion-generating system with salicylhydroxamic acid recovered ET production. In an in vitro experiment, superoxide anion was necessary and sufficient for conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ET because ET was produced from ACC solely in the presence of the superoxide-generating chemical KO2. ET production was also inhibited by lipoxygenase (LOX) inhibitors, indicating a possible involvement of LOX-mediated generation of superoxide anion and ET production itself. Furthermore, elicitin-induced ET production was completely inhibited by the protein synthesis inhibitor cycloheximide but recovered after exogenous application of ACC, indicating that de novo protein synthesis is required for ACC accumulation, leading to ET production. We also investigated the effects of several phytohormones on elicitor-induced ET production and discuss their role in the defense response.
| Original language | English |
|---|---|
| Pages (from-to) | 273-279 |
| Number of pages | 7 |
| Journal | Journal of General Plant Pathology |
| Volume | 71 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 2005 |
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Keywords
- Elicitin
- Ethylene
- Nicotiana tabacum
- Oxidative burst
ASJC Scopus subject areas
- Plant Science
Cite this
Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells. / Schenke, Dirk; Naito, Kana; Toyoda, Kazuhiro; Inagaki, Yoshishige; Shiraishi, Tomonori; Ichinose, Yuki.
In: Journal of General Plant Pathology, Vol. 71, No. 4, 08.2005, p. 273-279.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells
AU - Schenke, Dirk
AU - Naito, Kana
AU - Toyoda, Kazuhiro
AU - Inagaki, Yoshishige
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
PY - 2005/8
Y1 - 2005/8
N2 - INF1 elicitin, a proteinaceous elicitor produced by Phytophthora infestans, induces a hypersensitive response in tobacco BY-2 cells. In response to elicitin, tobacco cells produce both reactive oxygen species (ROS) and ethylene (ET). To investigate the regulation of elicitin-induced ET production, we pharmacologically analyzed the effects of several chemicals on ET production. Inhibitors of ROS generation or ROS chelators efficiently inhibited ET production, whereas simultaneous treatment of a superoxide anion-generating system with salicylhydroxamic acid recovered ET production. In an in vitro experiment, superoxide anion was necessary and sufficient for conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ET because ET was produced from ACC solely in the presence of the superoxide-generating chemical KO2. ET production was also inhibited by lipoxygenase (LOX) inhibitors, indicating a possible involvement of LOX-mediated generation of superoxide anion and ET production itself. Furthermore, elicitin-induced ET production was completely inhibited by the protein synthesis inhibitor cycloheximide but recovered after exogenous application of ACC, indicating that de novo protein synthesis is required for ACC accumulation, leading to ET production. We also investigated the effects of several phytohormones on elicitor-induced ET production and discuss their role in the defense response.
AB - INF1 elicitin, a proteinaceous elicitor produced by Phytophthora infestans, induces a hypersensitive response in tobacco BY-2 cells. In response to elicitin, tobacco cells produce both reactive oxygen species (ROS) and ethylene (ET). To investigate the regulation of elicitin-induced ET production, we pharmacologically analyzed the effects of several chemicals on ET production. Inhibitors of ROS generation or ROS chelators efficiently inhibited ET production, whereas simultaneous treatment of a superoxide anion-generating system with salicylhydroxamic acid recovered ET production. In an in vitro experiment, superoxide anion was necessary and sufficient for conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ET because ET was produced from ACC solely in the presence of the superoxide-generating chemical KO2. ET production was also inhibited by lipoxygenase (LOX) inhibitors, indicating a possible involvement of LOX-mediated generation of superoxide anion and ET production itself. Furthermore, elicitin-induced ET production was completely inhibited by the protein synthesis inhibitor cycloheximide but recovered after exogenous application of ACC, indicating that de novo protein synthesis is required for ACC accumulation, leading to ET production. We also investigated the effects of several phytohormones on elicitor-induced ET production and discuss their role in the defense response.
KW - Elicitin
KW - Ethylene
KW - Nicotiana tabacum
KW - Oxidative burst
UR - http://www.scopus.com/inward/record.url?scp=23944439109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944439109&partnerID=8YFLogxK
U2 - 10.1007/s10327-005-0197-0
DO - 10.1007/s10327-005-0197-0
M3 - Article
AN - SCOPUS:23944439109
VL - 71
SP - 273
EP - 279
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 4
ER -