Regulation of clathrin-mediated endocytosis by p53

Yoshie Endo, Atsumi Sugiyama, Shun Ai Li, Kazuji Ohmori, Hirokazu Ohata, Yusuke Yoshida, Masabumi Shibuya, Kohji Takei, Masato Enari, Yoichi Taya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.

Original languageEnglish
Pages (from-to)375-386
Number of pages12
JournalGenes to Cells
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 1 2008

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Endo, Y., Sugiyama, A., Li, S. A., Ohmori, K., Ohata, H., Yoshida, Y., Shibuya, M., Takei, K., Enari, M., & Taya, Y. (2008). Regulation of clathrin-mediated endocytosis by p53. Genes to Cells, 13(4), 375-386. https://doi.org/10.1111/j.1365-2443.2008.01172.x