Regulation of clathrin-mediated endocytosis by p53

Yoshie Endo, Atsumi Sugiyama, Shun Ai Li, Kazuji Ohmori, Hirokazu Ohata, Yusuke Yoshida, Masabumi Shibuya, Kohji Takei, Masato Enari, Yoichi Taya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.

Original languageEnglish
Pages (from-to)375-386
Number of pages12
JournalGenes to Cells
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Clathrin
Clathrin Heavy Chains
Endocytosis
Epidermal Growth Factor Receptor
p53 Genes
RNA Interference
Epidermal Growth Factor
Cytosol
Carcinogenesis
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Endo, Y., Sugiyama, A., Li, S. A., Ohmori, K., Ohata, H., Yoshida, Y., ... Taya, Y. (2008). Regulation of clathrin-mediated endocytosis by p53. Genes to Cells, 13(4), 375-386. https://doi.org/10.1111/j.1365-2443.2008.01172.x

Regulation of clathrin-mediated endocytosis by p53. / Endo, Yoshie; Sugiyama, Atsumi; Li, Shun Ai; Ohmori, Kazuji; Ohata, Hirokazu; Yoshida, Yusuke; Shibuya, Masabumi; Takei, Kohji; Enari, Masato; Taya, Yoichi.

In: Genes to Cells, Vol. 13, No. 4, 04.2008, p. 375-386.

Research output: Contribution to journalArticle

Endo, Y, Sugiyama, A, Li, SA, Ohmori, K, Ohata, H, Yoshida, Y, Shibuya, M, Takei, K, Enari, M & Taya, Y 2008, 'Regulation of clathrin-mediated endocytosis by p53', Genes to Cells, vol. 13, no. 4, pp. 375-386. https://doi.org/10.1111/j.1365-2443.2008.01172.x
Endo Y, Sugiyama A, Li SA, Ohmori K, Ohata H, Yoshida Y et al. Regulation of clathrin-mediated endocytosis by p53. Genes to Cells. 2008 Apr;13(4):375-386. https://doi.org/10.1111/j.1365-2443.2008.01172.x
Endo, Yoshie ; Sugiyama, Atsumi ; Li, Shun Ai ; Ohmori, Kazuji ; Ohata, Hirokazu ; Yoshida, Yusuke ; Shibuya, Masabumi ; Takei, Kohji ; Enari, Masato ; Taya, Yoichi. / Regulation of clathrin-mediated endocytosis by p53. In: Genes to Cells. 2008 ; Vol. 13, No. 4. pp. 375-386.
@article{be2592069e0840a2b8c5d5dbe82bba33,
title = "Regulation of clathrin-mediated endocytosis by p53",
abstract = "The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.",
author = "Yoshie Endo and Atsumi Sugiyama and Li, {Shun Ai} and Kazuji Ohmori and Hirokazu Ohata and Yusuke Yoshida and Masabumi Shibuya and Kohji Takei and Masato Enari and Yoichi Taya",
year = "2008",
month = "4",
doi = "10.1111/j.1365-2443.2008.01172.x",
language = "English",
volume = "13",
pages = "375--386",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Regulation of clathrin-mediated endocytosis by p53

AU - Endo, Yoshie

AU - Sugiyama, Atsumi

AU - Li, Shun Ai

AU - Ohmori, Kazuji

AU - Ohata, Hirokazu

AU - Yoshida, Yusuke

AU - Shibuya, Masabumi

AU - Takei, Kohji

AU - Enari, Masato

AU - Taya, Yoichi

PY - 2008/4

Y1 - 2008/4

N2 - The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.

AB - The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.

UR - http://www.scopus.com/inward/record.url?scp=41149154590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149154590&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2443.2008.01172.x

DO - 10.1111/j.1365-2443.2008.01172.x

M3 - Article

C2 - 18363968

AN - SCOPUS:41149154590

VL - 13

SP - 375

EP - 386

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 4

ER -