Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases

J. Miyaike, Yoshiaki Iwasaki, A. Takahashi, H. Shimomura, H. Taniguchi, N. Koide, K. Matsuura, T. Ogura, K. Tobe, T. Tsuji

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background and aim: Complement receptor type 1 (CR1) is a transmembrane protein, and human erythrocyte CR1 (E-CR1) is involved in the transport of circulating immune complexes (IC) from the circulation to the reticuloendothelial system, including the liver and spleen. In chronic viral hepatitis, increased levels of IC containing viral particles and an association with various extrahepatic manifestations have been reported. However, regulatory mechanisms for IC levels are not fully understood. Patients/subjects and methods: We analysed IC, E-CR1, and quantitative polymorphism of the CR1 gene in 149 patients with chronic viral liver diseases and in 64 normal blood donors using an enzyme linked immunosorbent assay, radioimmunoassay, and polymerase chain reaction-restriction fragment length polymorphism, respectively. We also analysed the effect of CR1 gene polymorphism on IC binding to E-CR1 using molecular methods. Results: E-CR1 levels in patients with chronic hepatitis and chronic viral liver diseases as a whole correlated inversely with increased levels of IC. Moreover, significantly high levels of IC were observed in patients with chronic hepatitis C (CH-C) who were homozygous for the E-CR1 low density allele. We also found low levels of E-CR1 in liver cirrhosis and CH-C but not in CH-B. Low levels of E-CR1 in CH-C were observed, even after considering the polymorphism of the CR1 gene. Finally, we demonstrated CR1 gene polymorphism dependent binding of hepatitis virus containing IC. Conclusions: Our results emphasise the important role of E-CR1 in clearance of IC from the circulation and the acquired, rather than inherited, decrease in E-CR1 in chronic viral liver diseases, especially of type C.

Original languageEnglish
Pages (from-to)591-596
Number of pages6
JournalGut
Volume51
Issue number4
DOIs
Publication statusPublished - Oct 2002

Fingerprint

Complement Receptors
Virus Diseases
Antigen-Antibody Complex
Liver Diseases
Erythrocytes
Chronic Hepatitis C
Chronic Hepatitis
Genes
Hepatitis Viruses
Mononuclear Phagocyte System
Blood Donors
Restriction Fragment Length Polymorphisms
Liver Cirrhosis
Virion
Radioimmunoassay
Spleen
Enzyme-Linked Immunosorbent Assay
Alleles
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases. / Miyaike, J.; Iwasaki, Yoshiaki; Takahashi, A.; Shimomura, H.; Taniguchi, H.; Koide, N.; Matsuura, K.; Ogura, T.; Tobe, K.; Tsuji, T.

In: Gut, Vol. 51, No. 4, 10.2002, p. 591-596.

Research output: Contribution to journalArticle

Miyaike, J, Iwasaki, Y, Takahashi, A, Shimomura, H, Taniguchi, H, Koide, N, Matsuura, K, Ogura, T, Tobe, K & Tsuji, T 2002, 'Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases', Gut, vol. 51, no. 4, pp. 591-596. https://doi.org/10.1136/gut.51.4.591
Miyaike, J. ; Iwasaki, Yoshiaki ; Takahashi, A. ; Shimomura, H. ; Taniguchi, H. ; Koide, N. ; Matsuura, K. ; Ogura, T. ; Tobe, K. ; Tsuji, T. / Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases. In: Gut. 2002 ; Vol. 51, No. 4. pp. 591-596.
@article{22ce6b41074247d5b3e9d44685e8540d,
title = "Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases",
abstract = "Background and aim: Complement receptor type 1 (CR1) is a transmembrane protein, and human erythrocyte CR1 (E-CR1) is involved in the transport of circulating immune complexes (IC) from the circulation to the reticuloendothelial system, including the liver and spleen. In chronic viral hepatitis, increased levels of IC containing viral particles and an association with various extrahepatic manifestations have been reported. However, regulatory mechanisms for IC levels are not fully understood. Patients/subjects and methods: We analysed IC, E-CR1, and quantitative polymorphism of the CR1 gene in 149 patients with chronic viral liver diseases and in 64 normal blood donors using an enzyme linked immunosorbent assay, radioimmunoassay, and polymerase chain reaction-restriction fragment length polymorphism, respectively. We also analysed the effect of CR1 gene polymorphism on IC binding to E-CR1 using molecular methods. Results: E-CR1 levels in patients with chronic hepatitis and chronic viral liver diseases as a whole correlated inversely with increased levels of IC. Moreover, significantly high levels of IC were observed in patients with chronic hepatitis C (CH-C) who were homozygous for the E-CR1 low density allele. We also found low levels of E-CR1 in liver cirrhosis and CH-C but not in CH-B. Low levels of E-CR1 in CH-C were observed, even after considering the polymorphism of the CR1 gene. Finally, we demonstrated CR1 gene polymorphism dependent binding of hepatitis virus containing IC. Conclusions: Our results emphasise the important role of E-CR1 in clearance of IC from the circulation and the acquired, rather than inherited, decrease in E-CR1 in chronic viral liver diseases, especially of type C.",
author = "J. Miyaike and Yoshiaki Iwasaki and A. Takahashi and H. Shimomura and H. Taniguchi and N. Koide and K. Matsuura and T. Ogura and K. Tobe and T. Tsuji",
year = "2002",
month = "10",
doi = "10.1136/gut.51.4.591",
language = "English",
volume = "51",
pages = "591--596",
journal = "Gut",
issn = "0017-5749",
publisher = "BMJ Publishing Group",
number = "4",

}

TY - JOUR

T1 - Regulation of circulating immune complexes by complement receptor type 1 on erythrocytes in chronic viral liver diseases

AU - Miyaike, J.

AU - Iwasaki, Yoshiaki

AU - Takahashi, A.

AU - Shimomura, H.

AU - Taniguchi, H.

AU - Koide, N.

AU - Matsuura, K.

AU - Ogura, T.

AU - Tobe, K.

AU - Tsuji, T.

PY - 2002/10

Y1 - 2002/10

N2 - Background and aim: Complement receptor type 1 (CR1) is a transmembrane protein, and human erythrocyte CR1 (E-CR1) is involved in the transport of circulating immune complexes (IC) from the circulation to the reticuloendothelial system, including the liver and spleen. In chronic viral hepatitis, increased levels of IC containing viral particles and an association with various extrahepatic manifestations have been reported. However, regulatory mechanisms for IC levels are not fully understood. Patients/subjects and methods: We analysed IC, E-CR1, and quantitative polymorphism of the CR1 gene in 149 patients with chronic viral liver diseases and in 64 normal blood donors using an enzyme linked immunosorbent assay, radioimmunoassay, and polymerase chain reaction-restriction fragment length polymorphism, respectively. We also analysed the effect of CR1 gene polymorphism on IC binding to E-CR1 using molecular methods. Results: E-CR1 levels in patients with chronic hepatitis and chronic viral liver diseases as a whole correlated inversely with increased levels of IC. Moreover, significantly high levels of IC were observed in patients with chronic hepatitis C (CH-C) who were homozygous for the E-CR1 low density allele. We also found low levels of E-CR1 in liver cirrhosis and CH-C but not in CH-B. Low levels of E-CR1 in CH-C were observed, even after considering the polymorphism of the CR1 gene. Finally, we demonstrated CR1 gene polymorphism dependent binding of hepatitis virus containing IC. Conclusions: Our results emphasise the important role of E-CR1 in clearance of IC from the circulation and the acquired, rather than inherited, decrease in E-CR1 in chronic viral liver diseases, especially of type C.

AB - Background and aim: Complement receptor type 1 (CR1) is a transmembrane protein, and human erythrocyte CR1 (E-CR1) is involved in the transport of circulating immune complexes (IC) from the circulation to the reticuloendothelial system, including the liver and spleen. In chronic viral hepatitis, increased levels of IC containing viral particles and an association with various extrahepatic manifestations have been reported. However, regulatory mechanisms for IC levels are not fully understood. Patients/subjects and methods: We analysed IC, E-CR1, and quantitative polymorphism of the CR1 gene in 149 patients with chronic viral liver diseases and in 64 normal blood donors using an enzyme linked immunosorbent assay, radioimmunoassay, and polymerase chain reaction-restriction fragment length polymorphism, respectively. We also analysed the effect of CR1 gene polymorphism on IC binding to E-CR1 using molecular methods. Results: E-CR1 levels in patients with chronic hepatitis and chronic viral liver diseases as a whole correlated inversely with increased levels of IC. Moreover, significantly high levels of IC were observed in patients with chronic hepatitis C (CH-C) who were homozygous for the E-CR1 low density allele. We also found low levels of E-CR1 in liver cirrhosis and CH-C but not in CH-B. Low levels of E-CR1 in CH-C were observed, even after considering the polymorphism of the CR1 gene. Finally, we demonstrated CR1 gene polymorphism dependent binding of hepatitis virus containing IC. Conclusions: Our results emphasise the important role of E-CR1 in clearance of IC from the circulation and the acquired, rather than inherited, decrease in E-CR1 in chronic viral liver diseases, especially of type C.

UR - http://www.scopus.com/inward/record.url?scp=0036785327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036785327&partnerID=8YFLogxK

U2 - 10.1136/gut.51.4.591

DO - 10.1136/gut.51.4.591

M3 - Article

VL - 51

SP - 591

EP - 596

JO - Gut

JF - Gut

SN - 0017-5749

IS - 4

ER -