Regulation of CCN1 via the 3′-untranslated region

Yosuke Nakagawa, Masanao Minato, Kumi Sumiyoshi, Azusa Maeda, Chikako Hara, Yurika Murase, Takashi Nishida, Satoshi Kubota, Masaharu Takigawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The 3′-untranslated region (UTR) is known to be a critical regulator of post-transcriptional events that determine the gene expression at the RNA level. The gene CCN1 is one of the classical members of the matricellular CCN family and is involved in a number of biological processes during mammalian development. In the present study, the 600-bp 3′-UTR of CCN1 was functionally characterized. Reporter gene analysis revealed that the entire 3′-UTR profoundly repressed gene expression in cis in different types of the cells, to which both the proximal and distal-halves of the 3′-UTR segments contributed almost equally. Deletion analysis of the 3′-UTR indicated a distinct functional element in the proximal half, whereas a putative target for microRNA-181s was predicted in silico in the distal half. Of note, the repressive RNA element in the proximal half was shown to be capable of forming a stable secondary structure. However, unexpectedly, a reporter construct with a tandem repeat of the predicted miR-181 targets failed to respond to miR-181a. In addition, the other major structured element predicted in the distal half was similarly characterized. To our surprise, the second element rather enhanced the reporter gene expression in cis. These results indicate the involvement of multiple regulatory elements in the CCN1 3′-UTR and suggest the complexity of the miRNA action as well as the 3′-UTR-mediated gene regulation.

Original languageEnglish
Pages (from-to)207-217
Number of pages11
JournalJournal of Cell Communication and Signaling
Volume7
Issue number3
DOIs
Publication statusPublished - Aug 2013

Fingerprint

3' Untranslated Regions
Gene expression
MicroRNAs
Reporter Genes
Gene Expression
Genes
RNA
Biological Phenomena
Tandem Repeat Sequences
Computer Simulation

Keywords

  • 3′-UTR
  • CCN family
  • CCN1
  • Cyr61
  • miRNA
  • Post-transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Regulation of CCN1 via the 3′-untranslated region. / Nakagawa, Yosuke; Minato, Masanao; Sumiyoshi, Kumi; Maeda, Azusa; Hara, Chikako; Murase, Yurika; Nishida, Takashi; Kubota, Satoshi; Takigawa, Masaharu.

In: Journal of Cell Communication and Signaling, Vol. 7, No. 3, 08.2013, p. 207-217.

Research output: Contribution to journalArticle

Nakagawa, Yosuke ; Minato, Masanao ; Sumiyoshi, Kumi ; Maeda, Azusa ; Hara, Chikako ; Murase, Yurika ; Nishida, Takashi ; Kubota, Satoshi ; Takigawa, Masaharu. / Regulation of CCN1 via the 3′-untranslated region. In: Journal of Cell Communication and Signaling. 2013 ; Vol. 7, No. 3. pp. 207-217.
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