Regulation of cancer-related growth factor expression by artificial zinc-finger proteins.

Tomoaki Mori, Jun Sasaki, Yasuhiro Aoyama, Takashi Sera

Research output: Contribution to journalArticle

Abstract

One attractive approach to anticancer therapy is repression of expression of vascular endothelial growth factor (VEGF) gene, which is a potent target for prevention of tumor growth. To achieve this, artificial transcription factors (ATF) designed for VEGF gene regulation were fused to cell-penetrating peptides (CPPs). We demonstrated ATFs fused to CPPs, designated CPP-ATFs or designed regulatory proteins (DRPs), could penetrate into mammalian cells and transiently repress expression of a reporter gene, which was under control of the VEGF promoter/5'-UTR. We discuss gene-regulatory properties of CPP-ATFs in detail.

Original languageEnglish
Pages (from-to)291-292
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number50
Publication statusPublished - 2006
Externally publishedYes

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Cell-Penetrating Peptides
Zinc Fingers
Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor A
Neoplasms
Proteins
5' Untranslated Regions
Regulator Genes
Reporter Genes
Genes
Transcription Factors
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Regulation of cancer-related growth factor expression by artificial zinc-finger proteins. / Mori, Tomoaki; Sasaki, Jun; Aoyama, Yasuhiro; Sera, Takashi.

In: Nucleic acids symposium series (2004), No. 50, 2006, p. 291-292.

Research output: Contribution to journalArticle

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