Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins

Yoshihiko Kobayashi, Yuki Yamamoto, Soichi Kageyama, Hiroki Hirayama, Koji Kimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nitric oxide (NO) is a regulator of sperm motility, oocyte/embryo survival, and waves of contraction/relaxation in mammalian oviducts. As follicles control oviductal functions by two routes at least, (1) a systemic way via blood vessels before ovulation, (2) a direct way by entering of follicular fluid through fimbria at ovulation, we hypothesized that NO synthesis in the bovine oviduct is regulated by follicular steroids and prostaglandins (PGs). Quantification of mRNA expressions in the ampullary tissues showed that inducible NO synthase (NOS2) mRNA expression was highest on the day of ovulation (day 0). By contrast, NOS2 mRNA expression in the isthmus was highest on days 5-6 and lowest on days 19-21. Endothelial NOS (NOS3) mRNA expressions in either the ampulla or the isthmus did not change during the estrous cycle. PGE2 and PGF2α increased NOS2 mRNA expressions in cultured ampullary oviductal epithelial cells after 1-h incubation. These increases were suppressed by an antagonist of E-prostanoid receptor type 2, one of the PGE2 receptor. Estradiol-17β decreased the expression of NOS2 mRNA expression in cultured isthmic epithelial cells 24 h after treatment. This effect was suppressed by an antagonist of estrogen receptor α (ESR1). Expression of ESR1 was highest on days 19-21 in the isthmic tissues. The overall findings indicate region-specific difference of NO synthesis in the oviduct. PGs flowed from ruptured follicle may up-regulate NO synthesis in the oviductal epithelium, whereas circulating E2 seems to inhibit NO synthesis via ESR1 in the isthmus at the follicular stage.

Original languageEnglish
Pages (from-to)577-587
Number of pages11
JournalReproduction
Volume151
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Prostaglandins
Nitric Oxide
Steroids
Messenger RNA
Ovulation
Oviducts
Epithelial Cells
Prostaglandin E Receptors
Follicular Fluid
Dinoprost
Estrous Cycle
Fallopian Tubes
Sperm Motility
Nitric Oxide Synthase Type II
Dinoprostone
Oocytes
Blood Vessels
Estradiol
Up-Regulation
Embryonic Structures

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins. / Kobayashi, Yoshihiko; Yamamoto, Yuki; Kageyama, Soichi; Hirayama, Hiroki; Kimura, Koji; Okuda, Kiyoshi.

In: Reproduction, Vol. 151, No. 6, 01.06.2016, p. 577-587.

Research output: Contribution to journalArticle

Kobayashi, Yoshihiko ; Yamamoto, Yuki ; Kageyama, Soichi ; Hirayama, Hiroki ; Kimura, Koji ; Okuda, Kiyoshi. / Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins. In: Reproduction. 2016 ; Vol. 151, No. 6. pp. 577-587.
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