Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum

Kanako Hayashi, Akio Miyamoto, Bajram Berisha, Michaela R. Kosmann, Kiyoshi Okuda, Dieter Schams

Research output: Contribution to journalArticle

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Abstract

Recent findings suggest that the ovarian renin-angiotensin system regulates ovarian function through the paracrine/autocrine actions of angiotensin (Ang) II. The aims of this study were to investigate 1) the endothelial cell capacity to convert Ang I to Ang II, 2) the effects of endocrine and paracrine/autocrine factors on Ang II production in microvascular endothelial cells (MVE) derived from the developing corpora lutea (CL), and 3) the relationship between Ang II peptide concentration and expression of mRNA for angiotensin type 1 and 2 receptors (ATR1 and AT2R) in the bovine CL at different stages of the estrous cycle. When Ang I was added to the MVE at a concentration of 10-9M, it was converted to Ang II (21%). The production of Ang II from Ang I time-dependently rose for 24 h. Addition of captopril (an inhibitor of Ang-converting enzyme [ACE]) to the MVE cultures significantly inhibited Ang II production from 6 h to 24 h (P <0.05). Addition of estradiol-17β (E2) + vascular endothelial growth factor and E2 + basic fibroblast growth factor to MVE cultures increased Ang II production, whereas E2 or growth factors alone had no effect. Specific transcription for AT1R and AT2R was detected in bovine CL and MVE. There were no significant changes in Ang II tissue concentration or AT1R mRNA expression using reverse transcription-polymerase chain reaction during the estrous cycle. In contrast, AT2R mRNA expression decreased during the midluteal phase (P <0.05) and increased to the highest level during the late luteal phase (P <0.05). Results demonstrated that Ang II is generated from Ang I in MVE isolated from the developing bovine CL, indicating that MVE have ACE activity. In addition, mRNA expression for Ang II receptors was detected in the bovine CL and the luteal MVE. These results suggest that Ang II is produced by actions of the local renin-angiotensin system, at least in part, on MVE in the bovine CL, and that this peptide may be involved in the regulation of luteal function during early development and luteolysis.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalBiology of Reproduction
Volume62
Issue number1
Publication statusPublished - 2000

Fingerprint

Angiotensin Receptors
Corpus Luteum
Angiotensin II
Endothelial Cells
Angiotensin I
Messenger RNA
Estrous Cycle
Renin-Angiotensin System
Cell Culture Techniques
Angiotensin Type 2 Receptor
Luteolysis
Angiotensin Type 1 Receptor
Peptides
Luteal Phase
Captopril
Fibroblast Growth Factor 2
Angiotensin-Converting Enzyme Inhibitors
Vascular Endothelial Growth Factor A
Reverse Transcription
Estradiol

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Hayashi, K., Miyamoto, A., Berisha, B., Kosmann, M. R., Okuda, K., & Schams, D. (2000). Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum. Biology of Reproduction, 62(1), 162-167.

Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum. / Hayashi, Kanako; Miyamoto, Akio; Berisha, Bajram; Kosmann, Michaela R.; Okuda, Kiyoshi; Schams, Dieter.

In: Biology of Reproduction, Vol. 62, No. 1, 2000, p. 162-167.

Research output: Contribution to journalArticle

Hayashi, K, Miyamoto, A, Berisha, B, Kosmann, MR, Okuda, K & Schams, D 2000, 'Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum', Biology of Reproduction, vol. 62, no. 1, pp. 162-167.
Hayashi, Kanako ; Miyamoto, Akio ; Berisha, Bajram ; Kosmann, Michaela R. ; Okuda, Kiyoshi ; Schams, Dieter. / Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum. In: Biology of Reproduction. 2000 ; Vol. 62, No. 1. pp. 162-167.
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