Abstract
The aim of the present work is to clarify the mechanism(s) that regulates the accumulation of protoporphyrin IX (PpIX) in human histiocytic lymphoma cell line U937 incubated with 5-aminolevulinic acid (ALA). Biosynthesis and accumulation of PpIX in the cells was determined after incubation with 0.1 ∼ 5 mM ALA using a flow cytometric technique. The synthesized endogenous PpIX was found to localize predominantly in the mitochondrial region of the cells. The ALA-enhanced PpIX synthesis was suppressed by the presence of either β-alanine, a competitive inhibitor of β-transporters on cell membranes, or carbonyl cyanide p-trifluoromethoxyphenyl hydrazone, an uncoupler of mitochondrial oxidative phosphorylation. In contrast, cellular accumulation of PpIX was enhanced by the presence of either deferoxamine (an iron chelater), MnCl2 (a ferrochelatase inhibitor), or Sn-mesoporphyrin (heme oxygenase inhibitor). These results suggest that ALA-enhanced accumulation of PpIX in U937 cells was regulated by cellular uptake and conversion of ALA to PpIX and by degradation of Heme.
| Original language | English |
|---|---|
| Pages (from-to) | 69-82 |
| Number of pages | 14 |
| Journal | Physiological chemistry and physics and medical NMR |
| Volume | 39 |
| Issue number | 1 |
| Publication status | Published - 2007 |
Keywords
- Aminolevulinic acid
- Ferrochelatase
- Heme oxygenase-1
- Protoporphyrin IX
- β-Transporters
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Physiology
- Spectroscopy