Regulation by nectin of the velocity of the formation of adherens junctions and tight junctions

Tomoyuki Honda, Kazuya Shimizu, Atsunori Fukuhara, Kenji Irie, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Cadherins are key Ca2+-dependent cell-cell adhesion molecules at adherens junctions (AJs) in fibroblasts and epithelial cells, whereas claudins are key Ca2+-independent cell-cell adhesion molecules at tight junctions (TJs) in epithelial cells. The formation and maintenance of TJs are dependent on the formation and maintenance of AJs. Nectins are Ca2+-independent immunoglobulin-like cell-cell adhesion molecules which comprise a family of four members, nectin-1, -2, -3, and -4, and are involved in the formation of AJs in cooperation with cadherins, and the subsequent formation of TJs. We show here that the velocity of the formation of the E-cadherin-based AJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in L cells stably expressing E-cadherin and Madin-Darby canine kidney cells. Moreover, the velocity of the formation of the claudin-based TJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in Madin-Darby canine kidney cells. These results indicate that nectins regulate the velocity of the formation of the E-cadherin-based AJs and the subsequent formation of the claudin-based TJs.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
Publication statusPublished - Jun 20 2003
Externally publishedYes

Keywords

  • Adherens junctions
  • Cadherin
  • Claudin
  • Nectin
  • Tight junctions

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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