Regulation and reversibility of vacuolar H+-ATPase

Tomoyuki Hirata, Norihiro Nakamura, Hiroshi Omote, Yoh Wada, Masamitsu Futai

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Arabidopsis thaliana vacuolar H+-translocating pyrophosphatase (V- PPase) was expressed funtionally in yeast vacuoles with endogenous vacuolar H+-ATPase (V-ATPase), and the regulation and reversibility of V-ATPase were studied using these vacuoles. Analysis of electrochemical proton gradient (ΔμH) formation with ATP and pyrophosphate indicated that the proton transport by V-ATPase or V-PPase is not regulated strictly by the proton chemical gradient (ΔpH. On the other hand, vacuolar membranes may have a regulatory mechanism for maintaining a constant membrane potential (ΔΨ). Chimeric vacuolar membranes showed ATP synthesis coupled with Δμh established by V-PPase. The ATP synthesis was sensitive to bafilomycin A1 and exhibited two apparent K(m) values for ADP. These results indicate that V-ATPase is a reversible enzyme. The ATP synthesis was not observed in the presence of nigericin, which dissipates ΔpH but not ΔΨ, suggesting that ΔpH is essential for ATP synthesis.

Original languageEnglish
Pages (from-to)386-389
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number1
DOIs
Publication statusPublished - Jan 7 2000
Externally publishedYes

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Vacuolar Proton-Translocating ATPases
Adenosine Triphosphate
Protons
Vacuoles
Membranes
Inorganic Pyrophosphatase
Nigericin
Pyrophosphatases
Proton-Motive Force
Arabidopsis
Membrane Potentials
Yeast
Adenosine Diphosphate
Yeasts
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation and reversibility of vacuolar H+-ATPase. / Hirata, Tomoyuki; Nakamura, Norihiro; Omote, Hiroshi; Wada, Yoh; Futai, Masamitsu.

In: Journal of Biological Chemistry, Vol. 275, No. 1, 07.01.2000, p. 386-389.

Research output: Contribution to journalArticle

Hirata, Tomoyuki ; Nakamura, Norihiro ; Omote, Hiroshi ; Wada, Yoh ; Futai, Masamitsu. / Regulation and reversibility of vacuolar H+-ATPase. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 1. pp. 386-389.
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