Regulated upon activation normal T-cell expressed and secreted (RANTES) contributes to abortion caused by Brucella abortus infection in pregnant mice

Kenta Watanabe, Natsumi Iwai, Masato Tachibana, Hidefumi Furuoka, Hiroshi Suzuki, Masahisa Watarai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Brucella abortus (B. abortus) is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant cells, and the causative agent of brucellosis. In the present study, we found that production of regulated upon activation normal T-cell expressed and secreted (RANTES) due to B. abortus infection contributes to abortion in pregnant mice. B. abortus infected pregnant interferon-γ (IFN-γ) knockout mice died within 15 days of infection, but non-pregnant IFN-γ knockout mice were still alive. With infection by wild type B. abortus, a large amount of RANTES production was observed in pregnant IFN-γ knockout mice, and induction of RANTES was also observed in normal pregnant mice infected with the wild type, but not in those infected with the intracellular replication-defective mutant. Production of RANTES and IFN-γ were inhibited in mice inoculated with the respective RANTES or IFN-γ antibody. Neutralization of RANTES, induced by B. abortus infection, served to prevent abortion. These results indicate that the production and function of RANTES are correlated with IFN-γ in pregnant mice infected with B. abortus.

Original languageEnglish
Pages (from-to)681-686
Number of pages6
JournalJournal of Veterinary Medical Science
Volume70
Issue number7
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Fingerprint

Brucella abortus
Brucella melitensis biovar Abortus
interferons
Interferons
T-lymphocytes
T-Lymphocytes
mice
Infection
infection
Knockout Mice
trophoblast
Brucellosis
brucellosis
Trophoblasts
giant cells
Giant Cells
Mouse
Activation
Abortion
neutralization

Keywords

  • Abortion
  • Brucella abortus
  • IFN-γ
  • RANTES

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

Regulated upon activation normal T-cell expressed and secreted (RANTES) contributes to abortion caused by Brucella abortus infection in pregnant mice. / Watanabe, Kenta; Iwai, Natsumi; Tachibana, Masato; Furuoka, Hidefumi; Suzuki, Hiroshi; Watarai, Masahisa.

In: Journal of Veterinary Medical Science, Vol. 70, No. 7, 07.2008, p. 681-686.

Research output: Contribution to journalArticle

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