Regionalized Gene Expression of Prolactin and Growth Hormone in the Chicken Anterior Pituitary Gland

Norio Kansaku, Kiyoshi Shimada, Noboru Saito

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Messenger ribonucleic acid (mRNA) levers of PRL and GH in perifused cephalic and caudal lobes of the anterior pituitary gland of the chicken were determined by Northern blot analysis and are related to hypothalamic peptide perifusion. Perifusion of vasoactive intestinal peptide (VIP, 10 and 100 nM) significantly increased both PRL secretion and levels of PRI mRNA in the cephalic lobe, whereas no effects of VIP were observed in the caudal lobe. In contrast, growth hormone-releasing factor (GRF) (1-100 nM) did not induce an increase in either GH secretion or levels of GH mRNA in the cephalic lobe but did increase GH secretion and levels of GH mRNA in the caudal lobe. In addition, forskolin perifusion increased secretion and levels of PRL and GH mRNA in both cephalic and caudal lobes, respectively. These results indicate that gene expression of PRL and GH in response to VIP and GRF, respectively, is localized to specific lobes of the anterior pituitary gland, but that they may share, in part, a common second messenger pathway, namely, cAMP.

Original languageEnglish
Pages (from-to)60-68
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume99
Issue number1
DOIs
Publication statusPublished - Jul 1995
Externally publishedYes

Fingerprint

anterior pituitary
Anterior Pituitary Gland
prolactin
Prolactin
somatotropin
Growth Hormone
Chickens
RNA
chickens
Gene Expression
gene expression
Head
secretion
somatoliberin
Growth Hormone-Releasing Hormone
vasoactive intestinal peptide
forskolin
second messengers
Vasoactive Intestinal Peptide
Second Messenger Systems

ASJC Scopus subject areas

  • Endocrinology

Cite this

Regionalized Gene Expression of Prolactin and Growth Hormone in the Chicken Anterior Pituitary Gland. / Kansaku, Norio; Shimada, Kiyoshi; Saito, Noboru.

In: General and Comparative Endocrinology, Vol. 99, No. 1, 07.1995, p. 60-68.

Research output: Contribution to journalArticle

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