Regional differences in the expression of DNA topoisomerase IIβ in the pyramidal neurons of the rat hippocampus

Yukihide Maeda, Kimiko Tsutsui, Ken Tsutsui, Akira Tokunaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalNeuroscience Research
Volume36
Issue number4
DOIs
Publication statusPublished - Apr 1 2000

Keywords

  • 3-D reconstruction
  • DNA topoisomerase IIβ
  • Hippocampal pyramidal cells
  • Immunohistochemistry
  • Postnatal development
  • Western blotting

ASJC Scopus subject areas

  • Neuroscience(all)

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