Regional changes in α-tubulin and β-actin mRNA accumulations after transient ischemia in spontaneously hypertensive rat brains

Y. Kondo, N. Ogawa, Masato Asanuma, H. Hirata, S. Nishibayashi, A. Mori

Research output: Contribution to journalArticle

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Abstract

Regional changes in the mRNA accumulations for cytoskeletal proteins α- tubulin and β-actin were examined by in situ hybridization and Northern blot analysis in spontaneously hypertensive rat brains at chronic stages after 3 hours of transient ischemia. α-Tubulin mRNA accumulations showed no significant change at 2 weeks after transient ischemia except for a significant decrease in the frontal cortex (9.7%, p <0.01) coinciding with ischemia induced histological changes. β-Actin mRNA level was significantly increased in the parietal cortex (8.5%), septum (10.0%), amygdala (11.0%), CA4 area (5.8%) and the dentate gyrus (7.5%) of the hippocampus at 2 weeks after recirculation compared with a sham-operated control group (p <0.01). The ischemic areas of hippocampal and frontocortical lesions receive afferent neurons from those regions where β-actin mRNA was increased, suggesting that ischemia-induced increases in β-actin mRNA may reflect actin synthesis in these neurons to compensate for lost synaptic connections. Two cytoskeletal mRNA concentrations reacted differently to cerebral ischemia, and did not parallel histological signs of ischemia either temporally or spatially.

Original languageEnglish
Pages (from-to)139-153
Number of pages15
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume86
Issue number2
Publication statusPublished - 1994
Externally publishedYes

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Inbred SHR Rats
Tubulin
Actins
Ischemia
Messenger RNA
Brain
Afferent Neurons
Parahippocampal Gyrus
Parietal Lobe
Cytoskeletal Proteins
Dentate Gyrus
Frontal Lobe
Amygdala
Brain Ischemia
Northern Blotting
In Situ Hybridization
Neurons
Control Groups

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Regional changes in α-tubulin and β-actin mRNA accumulations after transient ischemia in spontaneously hypertensive rat brains. / Kondo, Y.; Ogawa, N.; Asanuma, Masato; Hirata, H.; Nishibayashi, S.; Mori, A.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 86, No. 2, 1994, p. 139-153.

Research output: Contribution to journalArticle

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AU - Ogawa, N.

AU - Asanuma, Masato

AU - Hirata, H.

AU - Nishibayashi, S.

AU - Mori, A.

PY - 1994

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