Region-specific reduction of parvalbumin neurons and behavioral changes in adult mice following single exposure to cranial irradiation

Hiroshi Ueno, Shunsuke Suemitsu, Shinji Murakami, Naoya Kitamura, Kenta Wani, Yousuke Matsumoto, Motoi Okamoto, Takeshi Ishihara

Research output: Contribution to journalArticle

Abstract

Purpose: Ionizing irradiation has several long-term effects including progressive cognitive impairment. Cognitive deterioration generally appears to be caused by abnormalities in the hippocampal dentate gyrus, with abnormal function of parvalbumin-expressing interneurons (PV neurons) in the cerebral cortex. PV neurons are vulnerable to oxidative stress, which can be caused by ionizing irradiation. We speculated that selective impairment of specific brain regions due to ionizing irradiation may alter the degree of cognitive impairment. Methods: We irradiated mature mouse brains with 20 Gy-ionizing irradiation. Subsequently, we analyzed behavioral abnormalities and changes in the number of PV neurons. Results: PV neuron density was significantly lower in some cortical regions of irradiated mice than in control mice. Within 1 week of irradiation, both body weight and temperature of irradiated mice decreased. In the forced swim test, irradiated mice spent significantly less time immobile than did control mice. However, irradiated mice did not display any abnormalities in the elevated plus maze test, Y-maze test, tail suspension test, and social interaction test between 3 to 6 days after irradiation. Conclusions: These results suggest that high-dose irradiation is less likely to cause brain dysfunction in the subacute phase. Moreover, the vulnerability of PV neurons appears to be brain-region specific.

Original languageEnglish
JournalInternational Journal of Radiation Biology
DOIs
Publication statusPublished - Jan 1 2019

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Cranial Irradiation
Parvalbumins
Interneurons
Neurons
Brain
Hindlimb Suspension
Parahippocampal Gyrus
Dentate Gyrus
Interpersonal Relations
Body Temperature
Cerebral Cortex
Oxidative Stress
Body Weight

Keywords

  • Cognitive function
  • irradiation
  • oxidative stress
  • parvalbumin
  • perineuronal net

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Region-specific reduction of parvalbumin neurons and behavioral changes in adult mice following single exposure to cranial irradiation. / Ueno, Hiroshi; Suemitsu, Shunsuke; Murakami, Shinji; Kitamura, Naoya; Wani, Kenta; Matsumoto, Yousuke; Okamoto, Motoi; Ishihara, Takeshi.

In: International Journal of Radiation Biology, 01.01.2019.

Research output: Contribution to journalArticle

Ueno, Hiroshi ; Suemitsu, Shunsuke ; Murakami, Shinji ; Kitamura, Naoya ; Wani, Kenta ; Matsumoto, Yousuke ; Okamoto, Motoi ; Ishihara, Takeshi. / Region-specific reduction of parvalbumin neurons and behavioral changes in adult mice following single exposure to cranial irradiation. In: International Journal of Radiation Biology. 2019.
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