Regeneration of rat auditory ossicles using recombinant human BMP-2/collagen composites

Yasushi Takeda, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Noriyuki Nagai, Junko Yoshinobu, Mitsuhiro Okano, Kunihiro Fukushima, Ayako Takeuchi, Tadashi Yoshino, Kazunori Nishizaki

Research output: Contribution to journalArticle

7 Citations (Scopus)


Tympanoplasty operations to improve hearing impairment require a wide middle ear cavity and reconstruction of columellar formations. There is no specific material for use in the reconstruction of columellar formations. Tissue response to BMP has been employed as regenerative material. To our knowledge, however, there are no reports of the reconstruction of columellar formations using recombinant human bone morphogenetic protein-2/bovine collagen composites in the middle ear. The purpose of this study is to investigate whether recombinant human bone morphogenetic protein-2/bovine collagen composites (rhBMP-2 composites) are appropriate for use as regenerative material for tympanoplasty. In the form of pellets, rhBMP-2 composites were implanted as columellae into the tympanic cavity. At 2,4,6,8, and 12 weeks after surgery, the middle ear of the animals (n = 4 at each week) was stained with hematoxylineosin for light microscopic observation. All composites were in the process of ossification or had ossified according to their developmental stages and were covered with a single layer of squamous or cuboidal epithelium. The new bone formed in these composites was persistently stable and displayed some columellar conditions assessed by histological examination. This study led to the conclusion that rhBMP-2 composites make excellent regenerative material for auditory ossicles.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number2
Publication statusPublished - May 1 2005



  • Auditory ossicle
  • Columella
  • Recombinant human bone morphogenetic protein-2/bovine collagen composites (rhBMP-2 composites)
  • Regenerative material
  • Tympanic cavity

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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