Flagellar class 3 operons of Escherichia coli and Salmonella are transcribed by RNA polymerase containing σ28. The consensus sequence of the σ28-dependent promoters was believed to be TAAA N15 GCCGATAA. In this study, we found that the E. coli genome contains a large number of sequences homologous to this consensus. However, we showed that they do not always exert a σ28-dependent promoter activity. We compare more carefully the sequences of the class 3 flagellar promoters and propose a revised structure of the σ28-dependent promoters as TAAAGTTT N11 GCCGATAA.
ASJC Scopus subject areas
- Molecular Biology