Abstract
Background: Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both thrombolytic and anti-inflammatory effects, but its neuroprotective effects on cerebral ischemia are still unclear. The present study assessed the antioxidative and neurovascular unit (NVU) protective effects of SMTP-7 using transient middle cerebral artery occlusion (tMCAO) mice. Methods: After 60 minutes tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA + SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24 hours after reperfusion. We histologically assessed the hemorrhage and expressive changes of antioxidative markers in brains. Results: SMTP-7 treatment showed a similar antithrombotic effect to tPA, but significantly decreased the hemorrhage volumes and the number of 4-HNE, 3-NT and 8-OHdG positive cells, meanwhile, ameliorated the decrease of collagen IV in the ischemic brains. However, tPA + SMTP-7 treatment did not decrease hemorrhage volumes nor showed NVU protective effect. Conclusions: The present study suggested that SMTP-7 provided therapeutic benefits for ischemic stroke through antioxidative and NVU protective effects unlike tPA alone or tPA + SMTP-7.
| Original language | English |
|---|---|
| Journal | Journal of Stroke and Cerebrovascular Diseases |
| DOIs | |
| Publication status | Accepted/In press - Jan 1 2018 |
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Keywords
- Antioxidative
- ischemic stroke
- neurovascular unit
- SMTP-7
- Tpa
ASJC Scopus subject areas
- Surgery
- Rehabilitation
- Clinical Neurology
- Cardiology and Cardiovascular Medicine
Cite this
Reduction of Ischemia Reperfusion-Related Brain Hemorrhage by Stachybotrys Microspora Triprenyl Phenol-7 in Mice With Antioxidant Effects. / Huang, Yong; Ohta, Yasuyuki; Shang, Jingwei; Li, Xianghong; Liu, Xia; Shi, Xiaowen; Feng, Tian; Yamashita, Toru; Sato, Kota; Takemoto, Mami; Hishikawa, Nozomi; Suzuki, Eriko; Hasumi, Keiji; Abe, Koji.
In: Journal of Stroke and Cerebrovascular Diseases, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction of Ischemia Reperfusion-Related Brain Hemorrhage by Stachybotrys Microspora Triprenyl Phenol-7 in Mice With Antioxidant Effects
AU - Huang, Yong
AU - Ohta, Yasuyuki
AU - Shang, Jingwei
AU - Li, Xianghong
AU - Liu, Xia
AU - Shi, Xiaowen
AU - Feng, Tian
AU - Yamashita, Toru
AU - Sato, Kota
AU - Takemoto, Mami
AU - Hishikawa, Nozomi
AU - Suzuki, Eriko
AU - Hasumi, Keiji
AU - Abe, Koji
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Background: Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both thrombolytic and anti-inflammatory effects, but its neuroprotective effects on cerebral ischemia are still unclear. The present study assessed the antioxidative and neurovascular unit (NVU) protective effects of SMTP-7 using transient middle cerebral artery occlusion (tMCAO) mice. Methods: After 60 minutes tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA + SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24 hours after reperfusion. We histologically assessed the hemorrhage and expressive changes of antioxidative markers in brains. Results: SMTP-7 treatment showed a similar antithrombotic effect to tPA, but significantly decreased the hemorrhage volumes and the number of 4-HNE, 3-NT and 8-OHdG positive cells, meanwhile, ameliorated the decrease of collagen IV in the ischemic brains. However, tPA + SMTP-7 treatment did not decrease hemorrhage volumes nor showed NVU protective effect. Conclusions: The present study suggested that SMTP-7 provided therapeutic benefits for ischemic stroke through antioxidative and NVU protective effects unlike tPA alone or tPA + SMTP-7.
AB - Background: Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both thrombolytic and anti-inflammatory effects, but its neuroprotective effects on cerebral ischemia are still unclear. The present study assessed the antioxidative and neurovascular unit (NVU) protective effects of SMTP-7 using transient middle cerebral artery occlusion (tMCAO) mice. Methods: After 60 minutes tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA + SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24 hours after reperfusion. We histologically assessed the hemorrhage and expressive changes of antioxidative markers in brains. Results: SMTP-7 treatment showed a similar antithrombotic effect to tPA, but significantly decreased the hemorrhage volumes and the number of 4-HNE, 3-NT and 8-OHdG positive cells, meanwhile, ameliorated the decrease of collagen IV in the ischemic brains. However, tPA + SMTP-7 treatment did not decrease hemorrhage volumes nor showed NVU protective effect. Conclusions: The present study suggested that SMTP-7 provided therapeutic benefits for ischemic stroke through antioxidative and NVU protective effects unlike tPA alone or tPA + SMTP-7.
KW - Antioxidative
KW - ischemic stroke
KW - neurovascular unit
KW - SMTP-7
KW - Tpa
UR - http://www.scopus.com/inward/record.url?scp=85052962170&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052962170&partnerID=8YFLogxK
U2 - 10.1016/j.jstrokecerebrovasdis.2018.08.018
DO - 10.1016/j.jstrokecerebrovasdis.2018.08.018
M3 - Article
C2 - 30201460
AN - SCOPUS:85052962170
JO - Journal of Stroke and Cerebrovascular Diseases
JF - Journal of Stroke and Cerebrovascular Diseases
SN - 1052-3057
ER -