Reduction of Hg2+ with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1

Tsuyoshi Sugio, Mitsuko Fujii, Yumika Ninomiya, Tadayoshi Kanao, Atsunori Negishi, Fumiaki Takeuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1μM and 5μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33% by 5μM, and 70% by 10μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.

Original languageEnglish
Pages (from-to)1756-1763
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number7
DOIs
Publication statusPublished - 2008

Fingerprint

Acidithiobacillus
Acidithiobacillus ferrooxidans
cytochrome c
Electron Transport Complex IV
Cytochromes c
Mercury
mercury
cytochrome-c oxidase
Proteins
mercuric chloride
Mercuric Chloride
Electrons
Mercury (metal)
Vaporization
electrons
Volatilization
volatilization
Ions
Metals
Oxidoreductases

Keywords

  • Acidithiobacillus ferrooxidans
  • Cytochrome c oxidase
  • Iron oxidase
  • Mercury reduction
  • Mercury resistance

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science
  • Medicine(all)

Cite this

Reduction of Hg2+ with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1. / Sugio, Tsuyoshi; Fujii, Mitsuko; Ninomiya, Yumika; Kanao, Tadayoshi; Negishi, Atsunori; Takeuchi, Fumiaki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 7, 2008, p. 1756-1763.

Research output: Contribution to journalArticle

@article{31002e46abd345f49b86722a69b49219,
title = "Reduction of Hg2+ with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1",
abstract = "Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1μM and 5μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33{\%} by 5μM, and 70{\%} by 10μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.",
keywords = "Acidithiobacillus ferrooxidans, Cytochrome c oxidase, Iron oxidase, Mercury reduction, Mercury resistance",
author = "Tsuyoshi Sugio and Mitsuko Fujii and Yumika Ninomiya and Tadayoshi Kanao and Atsunori Negishi and Fumiaki Takeuchi",
year = "2008",
doi = "10.1271/bbb.80070",
language = "English",
volume = "72",
pages = "1756--1763",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "7",

}

TY - JOUR

T1 - Reduction of Hg2+ with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1

AU - Sugio, Tsuyoshi

AU - Fujii, Mitsuko

AU - Ninomiya, Yumika

AU - Kanao, Tadayoshi

AU - Negishi, Atsunori

AU - Takeuchi, Fumiaki

PY - 2008

Y1 - 2008

N2 - Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1μM and 5μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33% by 5μM, and 70% by 10μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.

AB - Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1μM and 5μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33% by 5μM, and 70% by 10μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.

KW - Acidithiobacillus ferrooxidans

KW - Cytochrome c oxidase

KW - Iron oxidase

KW - Mercury reduction

KW - Mercury resistance

UR - http://www.scopus.com/inward/record.url?scp=47949083772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47949083772&partnerID=8YFLogxK

U2 - 10.1271/bbb.80070

DO - 10.1271/bbb.80070

M3 - Article

C2 - 18603796

AN - SCOPUS:47949083772

VL - 72

SP - 1756

EP - 1763

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 7

ER -