Reduction of hematopoietic cell-specific tyrosine phosphatase SHP-1 gene expression in natural killer cell lymphoma and various types of lymphomas/leukemias: Combination Analysis with cDNA expression array and tissue microarray

Takashi Oka, Tadashi Yoshino, K. Hayashi, N. Ohara, T. Nakanishi, Y. Yamaai, A. Hiraki, Chiharu Sogawa, E. Kondo, N. Teramoto, K. Takahashi, J. Tsuchiyama, T. Akagi

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Abstract

To investigate the lymphomagenesis of NK/T lymphoma, we comprehensively and systematically analyzed the expression pattern of the human NK/T cell line (NK-YS) genome by cDNA expression array and tissue microarray. We detected significant changes in the gene expression of NK-YS cell line: an increase in 18 and a decrease in 20 genes compared to normal NK cells or peripheral blood mononuclear cells. Among these genes, we found a strong decrease in hematopoietic cell specific protein-tyrosine-phosphatase SH-PTP1 (SHP1) mRNA by cDNA expression array and reverse transcriptase-polymerase chain reaction. Further analysis with standard immunohistochemistry and tissue microarray, which used 207 paraffin-embedded specimens of various kinds of malignant lymphomas, showed that 100% of NK/T lymphoma specimens and more than 95% of various types of malignant lymphoma were negative for SHP1 protein expression. On the other hand, SHP1 protein was strongly expressed in the mantle zone and interfollicular zone lymphocytes in reactive lymphoid hyperplasia specimens. In addition, various kinds of hematopoietic cell lines, particularly the highly aggressive lymphoma/leukemia lines, lacked SHP1 expression in vitro, suggesting that loss of SHP1 expression may be related to not only malignant transformation, but also tumor cell aggressiveness. SHP1 expression could not be induced in either of two NK/T cell lines by phorbol ester, suggesting that genetic impairment or modification with methylation of SHP1 DNA could be one of the critical events in the pathogenesis of NK/T lymphoma. This evidence strongly suggests that loss of SHP1 gene expression plays an important role in multistep tumorigenesis, possibly as an anti-oncogene in the wide range of lymphomas/leukemias as well as NK/T lymphomas.

Original languageEnglish
Pages (from-to)1495-1505
Number of pages11
JournalAmerican Journal of Pathology
Volume159
Issue number4
Publication statusPublished - 2001

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Non-Receptor Type 6 Protein Tyrosine Phosphatase
Oligonucleotide Array Sequence Analysis
Natural Killer Cells
Lymphoma
Leukemia
Gene Expression
Cell Line
Pseudolymphoma
T-Lymphocytes
Protein Tyrosine Phosphatases
Phorbol Esters
Tumor Suppressor Genes
Reverse Transcriptase Polymerase Chain Reaction
Paraffin
Methylation
Genes
Blood Cells
Carcinogenesis
Proteins
Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Reduction of hematopoietic cell-specific tyrosine phosphatase SHP-1 gene expression in natural killer cell lymphoma and various types of lymphomas/leukemias : Combination Analysis with cDNA expression array and tissue microarray. / Oka, Takashi; Yoshino, Tadashi; Hayashi, K.; Ohara, N.; Nakanishi, T.; Yamaai, Y.; Hiraki, A.; Sogawa, Chiharu; Kondo, E.; Teramoto, N.; Takahashi, K.; Tsuchiyama, J.; Akagi, T.

In: American Journal of Pathology, Vol. 159, No. 4, 2001, p. 1495-1505.

Research output: Contribution to journalArticle

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abstract = "To investigate the lymphomagenesis of NK/T lymphoma, we comprehensively and systematically analyzed the expression pattern of the human NK/T cell line (NK-YS) genome by cDNA expression array and tissue microarray. We detected significant changes in the gene expression of NK-YS cell line: an increase in 18 and a decrease in 20 genes compared to normal NK cells or peripheral blood mononuclear cells. Among these genes, we found a strong decrease in hematopoietic cell specific protein-tyrosine-phosphatase SH-PTP1 (SHP1) mRNA by cDNA expression array and reverse transcriptase-polymerase chain reaction. Further analysis with standard immunohistochemistry and tissue microarray, which used 207 paraffin-embedded specimens of various kinds of malignant lymphomas, showed that 100{\%} of NK/T lymphoma specimens and more than 95{\%} of various types of malignant lymphoma were negative for SHP1 protein expression. On the other hand, SHP1 protein was strongly expressed in the mantle zone and interfollicular zone lymphocytes in reactive lymphoid hyperplasia specimens. In addition, various kinds of hematopoietic cell lines, particularly the highly aggressive lymphoma/leukemia lines, lacked SHP1 expression in vitro, suggesting that loss of SHP1 expression may be related to not only malignant transformation, but also tumor cell aggressiveness. SHP1 expression could not be induced in either of two NK/T cell lines by phorbol ester, suggesting that genetic impairment or modification with methylation of SHP1 DNA could be one of the critical events in the pathogenesis of NK/T lymphoma. This evidence strongly suggests that loss of SHP1 gene expression plays an important role in multistep tumorigenesis, possibly as an anti-oncogene in the wide range of lymphomas/leukemias as well as NK/T lymphomas.",
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AU - Oka, Takashi

AU - Yoshino, Tadashi

AU - Hayashi, K.

AU - Ohara, N.

AU - Nakanishi, T.

AU - Yamaai, Y.

AU - Hiraki, A.

AU - Sogawa, Chiharu

AU - Kondo, E.

AU - Teramoto, N.

AU - Takahashi, K.

AU - Tsuchiyama, J.

AU - Akagi, T.

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