Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270

T. M. Taha, F. Takeuchi, T. Sugio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is mysterious that, when A. ferrooxidans ATCC 23270 cells grow on elemental sulfur, they have high iron oxidase activity comparable to that of iron-grown cells as well as high activities of sulfide:ferric ion oxidoreductase (SFORase) and tetrathionate hydrolase. To clarify this interesting phenomenon, cytochrome c and tetrathionate hydrolase were purified from sulfur-grown A. ferrooxidans cells using ammonium sulfate precipitation, Phenyl column chromatography, and Superdex™ 75 and Sephadex G-100 size exclusion column chromatographies. The purified cytochrome c was reduced by tetrathionate in the presence of purified tetrathionate hydrolase, but not in the absence of the enzyme. When the partially purified cytochrome c fraction containing aa 3-type cytochrome oxidase was used, both cytochrome c and aa 3-type cytochrome oxidase were reduced by tetrathionate in the presence of purified tetrathionate hydrolase. These results indicate that tetrathionate in the presence of tetrathionate hydrolase can reduce iron oxidase enzyme system containing cytochrome c and aa3-type cytochrome oxidase as tetrathionate hydrolase decomposes tetrathionate to produce thiosulfate, elemental sulfur, and sulfate; and the formed thiosulfate can chemically reduce cytochrome c and Fe3+.

Original languageEnglish
Title of host publicationBiohydrometallurgy 2009
Subtitle of host publicationA Meeting Point between Microbial Ecology, Metal Recovery Processes and Environmental Remediation - Selected, peer rev. papers 18th Int. Biohydrometallurgy Symp., IBS 2009
Pages243-246
Number of pages4
DOIs
Publication statusPublished - Dec 28 2009
Event18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duration: Sep 13 2009Sep 17 2009

Publication series

NameAdvanced Materials Research
Volume71-73
ISSN (Print)1022-6680

Other

Other18th International Biohydrometallurgy Symposium, IBS 2009
CountryArgentina
CityBariloche
Period9/13/099/17/09

Keywords

  • Cytochrome reduction
  • Electron transport chain
  • Sulfur oxidation
  • Tetrathionate hydrolase

ASJC Scopus subject areas

  • Engineering(all)

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    Taha, T. M., Takeuchi, F., & Sugio, T. (2009). Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270. In Biohydrometallurgy 2009: A Meeting Point between Microbial Ecology, Metal Recovery Processes and Environmental Remediation - Selected, peer rev. papers 18th Int. Biohydrometallurgy Symp., IBS 2009 (pp. 243-246). (Advanced Materials Research; Vol. 71-73). https://doi.org/10.4028/www.scientific.net/AMR.71-73.243