Reduced permeability to K+ and Na+ ions of K+ channels in the plasma membrane of tobacco cells in suspension after adaptation to 50 mM NaCl

Yoshiyuki Murata, Ichiro Obi, Manabu Yoshihashi, Masakatsu Noguchi, Tadaaki Kakutani

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The whole-cell patch-clamp technique was used to study and compare the characteristics of K+-and Na+-transport processes across the plasma membrane in two types of protoplast isolated from NaCl-adapted and -unadapted cells of tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) in suspension culture. In both types of protoplast, with 100 mM KCl in the bathing solution and in the pipette solution, depolarization of the plasma membrane from the holding potential of 0 mV to a positive potential resulted in a relatively large outward current which increased with increasing positive potential, whereas hyperpolarization to negative potentials up to -100 mV resulted in only a small inward current. The outward current activated by depolarization was predominantly carried by K+ ions through K+ channels. Na+ ions also had a finite ability to pass through these K+ channels. The outward K+ and Na+ currents of the NaCl-adapted cells were considerably smaller than those of the NaCl-unadapted cells. These results suggest that adaptation to salinity results in reduced permeability of the plasma membrane to both K+ and Na+ ions.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalPlant and Cell Physiology
Volume35
Issue number1
Publication statusPublished - Dec 1 1994
Externally publishedYes

Keywords

  • K+ channel
  • Patch clamp
  • Plasma membrane
  • Salt stress
  • Tobacco suspension cell

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Reduced permeability to K<sup>+</sup> and Na<sup>+</sup> ions of K<sup>+</sup> channels in the plasma membrane of tobacco cells in suspension after adaptation to 50 mM NaCl'. Together they form a unique fingerprint.

  • Cite this