Reduced permeability to K+ and Na+ ions of K+ channels in the plasma membrane of tobacco cells in suspension after adaptation to 50 mM NaCl

Yoshiyuki Murata, Ichiro Obi, Manabu Yoshihashi, Masakatsu Noguchi, Tadaaki Kakutani

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The whole-cell patch-clamp technique was used to study and compare the characteristics of K+-and Na+-transport processes across the plasma membrane in two types of protoplast isolated from NaCl-adapted and -unadapted cells of tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) in suspension culture. In both types of protoplast, with 100 mM KCl in the bathing solution and in the pipette solution, depolarization of the plasma membrane from the holding potential of 0 mV to a positive potential resulted in a relatively large outward current which increased with increasing positive potential, whereas hyperpolarization to negative potentials up to -100 mV resulted in only a small inward current. The outward current activated by depolarization was predominantly carried by K+ ions through K+ channels. Na+ ions also had a finite ability to pass through these K+ channels. The outward K+ and Na+ currents of the NaCl-adapted cells were considerably smaller than those of the NaCl-unadapted cells. These results suggest that adaptation to salinity results in reduced permeability of the plasma membrane to both K+ and Na+ ions.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalPlant and Cell Physiology
Volume35
Issue number1
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

K+ Channel
Plasma Membrane
Tobacco
potassium channels
Cell membranes
tobacco
Ion Channels
Permeability
cell suspension culture
Suspensions
permeability
plasma membrane
Depolarization
Cell Membrane
ions
membrane
plasma
Protoplast
ion
Protoplasts

Keywords

  • K+ channel
  • Patch clamp
  • Plasma membrane
  • Salt stress
  • Tobacco suspension cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Reduced permeability to K+ and Na+ ions of K+ channels in the plasma membrane of tobacco cells in suspension after adaptation to 50 mM NaCl. / Murata, Yoshiyuki; Obi, Ichiro; Yoshihashi, Manabu; Noguchi, Masakatsu; Kakutani, Tadaaki.

In: Plant and Cell Physiology, Vol. 35, No. 1, 1994, p. 87-92.

Research output: Contribution to journalArticle

Murata, Yoshiyuki ; Obi, Ichiro ; Yoshihashi, Manabu ; Noguchi, Masakatsu ; Kakutani, Tadaaki. / Reduced permeability to K+ and Na+ ions of K+ channels in the plasma membrane of tobacco cells in suspension after adaptation to 50 mM NaCl. In: Plant and Cell Physiology. 1994 ; Vol. 35, No. 1. pp. 87-92.
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