Reduced expression of insulin-like growth factor binding protein-3 and its promoter hypermethylation in human hepatocellular carcinoma

Tadashi Hanafusa, Yasuhiro Yumoto, Kazuhiro Nouso, Harushige Nakatsukasa, Toru Onishi, Tatsuya Fujikawa, Mayumi Taniyama, Shinichiro Nakamura, Masayuki Uemura, Yoshitaka Takuma, Eiichiro Yumoto, Toshihiro Higashi, Takao Tsuji

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) is postulated to be a mediator of growth suppression signals. Reduced expression of the IGFBP-3 was observed in nine out of 12 human hepatocellular carcinomas (HCC) (75%). Promoter hypermethylation of the IGFBP-3 was detected in four out of 12 HCCs (33%) although mutations were not identified. The expression of IGFBP-3 was restored by the demethylating agent 5-aza-2′-deoxycytidine in HCC cell line with promoter hypermethylation (HepG2). As IGFBP-3 functions like a tumor suppressor gene, it may be used as a therapeutic target for HCC.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalCancer Letters
Volume176
Issue number2
DOIs
Publication statusPublished - Feb 25 2002

Keywords

  • Hepatocellular carcinoma
  • Hypermethylation
  • Insulin-like growth factor binding protein-3
  • Mannose 6-phosphate/insulin-like growth factor 2 receptor
  • cDNA microarray

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Hanafusa, T., Yumoto, Y., Nouso, K., Nakatsukasa, H., Onishi, T., Fujikawa, T., Taniyama, M., Nakamura, S., Uemura, M., Takuma, Y., Yumoto, E., Higashi, T., & Tsuji, T. (2002). Reduced expression of insulin-like growth factor binding protein-3 and its promoter hypermethylation in human hepatocellular carcinoma. Cancer Letters, 176(2), 149-158. https://doi.org/10.1016/S0304-3835(01)00736-4