Redox regulation of Ca2+/calmodulin-dependent protein kinase IV via oxidation of its active-site cysteine residue

Tsuyoshi Takata, Jun Kimura, Hideshi Ihara, Naoya Hatano, Yukihiro Tsuchiya, Yasuo Watanabe

Research output: Contribution to journalArticle

Abstract

We have recently reported that Ca2+/calmodulin (CaM)-dependent protein kinase IV (CaMKIV) is inactivated by reactive sulfur species via polysulfidation of the active-site Cys residue. Here, we show that hydrogen peroxide (H2O2) limit CaMKIV activity at the same active-site Cys residue through oxidation and downstream signaling in cells. CaMKIV is phosphorylated at Thr196 by its upstream CaMK kinase (CaMKK), which induces its full activity. In vitro incubation of CaMKIV with H2O2 resulted in reversible inhibition of CaMKK-induced phospho-Thr196 and the consequent inactivation of CaMKIV. In contrast, mutated CaMKIV (C198V) was refractory to the H2O2-induced enzyme inhibition. In transfected cells expressing CaMKIV, Ca2+ ionophore-induced CaMKIV phosphorylation at Thr196 was decreased upon treatment with H2O2, whereas cells expressing mutant CaMKIV (C198V) were resistant to H2O2 treatment. Modification of free thiol with N-ethylmaleimide revealed that Cys198 in CaMKIV is a target for S-oxidation. Additionally, the Ca2+ influx-induced phospho-Thr196 of endogenous CaMKIV was also inhibited upon treatment with H2O2 in Jurkat T-lymphocytes and cerebellar granule cells. Phosphorylation of cyclic AMP response element-binding protein (CREB) at Ser133, which is downstream of CaMKIV, was also decreased upon treatment with H2O2. Thus, our results indicate that oxidation stress regulates cellular function by decreasing the activity of CaMKIV through Cys198 oxidation.

LanguageEnglish
Pages99-106
Number of pages8
JournalFree Radical Biology and Medicine
Volume130
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 4
Protein Kinases
Oxidation-Reduction
Cysteine
Catalytic Domain
Oxidation
Phosphorylation
Phosphotransferases
Cells
Enzyme inhibition
Cyclic AMP Response Element-Binding Protein
Ethylmaleimide
T-cells
Ionophores

Keywords

  • Ca/calmodulin-dependent protein kinase (CaMK)
  • Phosphorylation
  • Redox regulation
  • S-oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Redox regulation of Ca2+/calmodulin-dependent protein kinase IV via oxidation of its active-site cysteine residue. / Takata, Tsuyoshi; Kimura, Jun; Ihara, Hideshi; Hatano, Naoya; Tsuchiya, Yukihiro; Watanabe, Yasuo.

In: Free Radical Biology and Medicine, Vol. 130, 01.01.2019, p. 99-106.

Research output: Contribution to journalArticle

Takata, Tsuyoshi ; Kimura, Jun ; Ihara, Hideshi ; Hatano, Naoya ; Tsuchiya, Yukihiro ; Watanabe, Yasuo. / Redox regulation of Ca2+/calmodulin-dependent protein kinase IV via oxidation of its active-site cysteine residue. In: Free Radical Biology and Medicine. 2019 ; Vol. 130. pp. 99-106.
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