Red blood cell coagulation induced by low-temperature plasma treatment

Kenji Miyamoto, Sanae Ikehara, Hikaru Takei, Yoshihiro Akimoto, Hajime Sakakita, Kenji Ishikawa, Masashi Ueda, Jun-Ichiro Ikeda, Masahiro Yamagishi, Jaeho Kim, Takashi Yamaguchi, Hayao Nakanishi, Tetsuji Shimizu, Nobuyuki Shimizu, Masaru Hori, Yuzuru Ikehara

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.

Original languageEnglish
JournalArchives of Biochemistry and Biophysics
DOIs
Publication statusPublished - Mar 28 2016
Externally publishedYes

Fingerprint

Blood Coagulation
Coagulation
Blood
Erythrocytes
Cells
Plasmas
Temperature
Hemolysis
Thrombosis
Membranes
Phosphates
Electrons
Blood Coagulation Factors
Platelet Aggregation
Platelets
Blood Proteins
Carrier concentration
Light
Equipment and Supplies

Cite this

Miyamoto, K., Ikehara, S., Takei, H., Akimoto, Y., Sakakita, H., Ishikawa, K., ... Ikehara, Y. (2016). Red blood cell coagulation induced by low-temperature plasma treatment. Archives of Biochemistry and Biophysics. https://doi.org/10.1016/j.abb.2016.03.023

Red blood cell coagulation induced by low-temperature plasma treatment. / Miyamoto, Kenji; Ikehara, Sanae; Takei, Hikaru; Akimoto, Yoshihiro; Sakakita, Hajime; Ishikawa, Kenji; Ueda, Masashi; Ikeda, Jun-Ichiro; Yamagishi, Masahiro; Kim, Jaeho; Yamaguchi, Takashi; Nakanishi, Hayao; Shimizu, Tetsuji; Shimizu, Nobuyuki; Hori, Masaru; Ikehara, Yuzuru.

In: Archives of Biochemistry and Biophysics, 28.03.2016.

Research output: Contribution to journalArticle

Miyamoto, K, Ikehara, S, Takei, H, Akimoto, Y, Sakakita, H, Ishikawa, K, Ueda, M, Ikeda, J-I, Yamagishi, M, Kim, J, Yamaguchi, T, Nakanishi, H, Shimizu, T, Shimizu, N, Hori, M & Ikehara, Y 2016, 'Red blood cell coagulation induced by low-temperature plasma treatment', Archives of Biochemistry and Biophysics. https://doi.org/10.1016/j.abb.2016.03.023
Miyamoto, Kenji ; Ikehara, Sanae ; Takei, Hikaru ; Akimoto, Yoshihiro ; Sakakita, Hajime ; Ishikawa, Kenji ; Ueda, Masashi ; Ikeda, Jun-Ichiro ; Yamagishi, Masahiro ; Kim, Jaeho ; Yamaguchi, Takashi ; Nakanishi, Hayao ; Shimizu, Tetsuji ; Shimizu, Nobuyuki ; Hori, Masaru ; Ikehara, Yuzuru. / Red blood cell coagulation induced by low-temperature plasma treatment. In: Archives of Biochemistry and Biophysics. 2016.
@article{273fccf376054b2686d18aefd80ff4ca,
title = "Red blood cell coagulation induced by low-temperature plasma treatment",
abstract = "Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.",
author = "Kenji Miyamoto and Sanae Ikehara and Hikaru Takei and Yoshihiro Akimoto and Hajime Sakakita and Kenji Ishikawa and Masashi Ueda and Jun-Ichiro Ikeda and Masahiro Yamagishi and Jaeho Kim and Takashi Yamaguchi and Hayao Nakanishi and Tetsuji Shimizu and Nobuyuki Shimizu and Masaru Hori and Yuzuru Ikehara",
note = "Copyright {\circledC} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = "3",
day = "28",
doi = "10.1016/j.abb.2016.03.023",
language = "English",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Red blood cell coagulation induced by low-temperature plasma treatment

AU - Miyamoto, Kenji

AU - Ikehara, Sanae

AU - Takei, Hikaru

AU - Akimoto, Yoshihiro

AU - Sakakita, Hajime

AU - Ishikawa, Kenji

AU - Ueda, Masashi

AU - Ikeda, Jun-Ichiro

AU - Yamagishi, Masahiro

AU - Kim, Jaeho

AU - Yamaguchi, Takashi

AU - Nakanishi, Hayao

AU - Shimizu, Tetsuji

AU - Shimizu, Nobuyuki

AU - Hori, Masaru

AU - Ikehara, Yuzuru

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/3/28

Y1 - 2016/3/28

N2 - Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.

AB - Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.

U2 - 10.1016/j.abb.2016.03.023

DO - 10.1016/j.abb.2016.03.023

M3 - Article

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

ER -