TY - JOUR
T1 - Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers
AU - Shiraga, Kaori
AU - Soe, Tet Htut
AU - Matsumoto, Sho
AU - Watanabe, Kazunori
AU - Ohtsuki, Takashi
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number 26107711 and 18H02090 (to T. O.).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/9/19
Y1 - 2018/9/19
N2 - Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.
AB - Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.
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U2 - 10.1021/acs.bioconjchem.8b00487
DO - 10.1021/acs.bioconjchem.8b00487
M3 - Article
C2 - 30063334
AN - SCOPUS:85052316100
SN - 1043-1802
VL - 29
SP - 3174
EP - 3179
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 9
ER -