Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers

Kaori Shiraga, Tet Htut Soe, Sho Matsumoto, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.

Original languageEnglish
Pages (from-to)3174-3179
Number of pages6
JournalBioconjugate Chemistry
Volume29
Issue number9
DOIs
Publication statusPublished - Sep 19 2018

Fingerprint

RNA
Infrared radiation
Light
Up-Regulation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers. / Shiraga, Kaori; Soe, Tet Htut; Matsumoto, Sho; Watanabe, Kazunori; Ohtsuki, Takashi.

In: Bioconjugate Chemistry, Vol. 29, No. 9, 19.09.2018, p. 3174-3179.

Research output: Contribution to journalArticle

@article{e4178cad2e6b4e7eb7913b3964b7c84e,
title = "Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers",
abstract = "Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.",
author = "Kaori Shiraga and Soe, {Tet Htut} and Sho Matsumoto and Kazunori Watanabe and Takashi Ohtsuki",
year = "2018",
month = "9",
day = "19",
doi = "10.1021/acs.bioconjchem.8b00487",
language = "English",
volume = "29",
pages = "3174--3179",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers

AU - Shiraga, Kaori

AU - Soe, Tet Htut

AU - Matsumoto, Sho

AU - Watanabe, Kazunori

AU - Ohtsuki, Takashi

PY - 2018/9/19

Y1 - 2018/9/19

N2 - Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.

AB - Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.

UR - http://www.scopus.com/inward/record.url?scp=85052316100&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052316100&partnerID=8YFLogxK

U2 - 10.1021/acs.bioconjchem.8b00487

DO - 10.1021/acs.bioconjchem.8b00487

M3 - Article

C2 - 30063334

AN - SCOPUS:85052316100

VL - 29

SP - 3174

EP - 3179

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 9

ER -