TY - JOUR
T1 - Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan
AU - Ujihara, Takako
AU - Uchiyama, Jumpei
AU - Nasukawa, Tadahiro
AU - Ando, Hiroki
AU - Murakami, Hironobu
AU - Ohara, Naoya
AU - Ogawa, Midori
AU - Yamazaki, Toshio
AU - Daibata, Masanori
AU - Sakaguchi, Masahiro
AU - Matsuzaki, Shigenobu
N1 - Funding Information:
Acknowledgements The authors thank the staff of the Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi, Japan, for experimental advice. They also thank Mr. Hiroki Tsurui and Mr. Masaya Ogata, Azabu University, Kanagawa, Japan, for their technical help. This research was supported by Research Funding Granted by Kochi University President, and by Research Funding Granted by Azabu University.
Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
AB - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
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U2 - 10.1007/s00705-018-3788-8
DO - 10.1007/s00705-018-3788-8
M3 - Article
C2 - 29511830
AN - SCOPUS:85048470792
VL - 163
SP - 1915
EP - 1919
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 7
ER -