Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

Takako Ujihara; Jumpei Uchiyama; Tadahiro Nasukawa; Hiroki Ando; Hironobu Murakami; Naoya Ohara; Midori Ogawa; Toshio Yamazaki; Masanori Daibata; Masahiro Sakaguchi; Shigenobu Matsuzaki

doi:10.1007/s00705-018-3788-8

Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

Takako Ujihara, Jumpei Uchiyama, Tadahiro Nasukawa, Hiroki Ando, Hironobu Murakami, Naoya Ohara, Midori Ogawa, Toshio Yamazaki, Masanori Daibata, Masahiro Sakaguchi, Shigenobu Matsuzaki

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

Original language	English
Pages (from-to)	1915-1919
Number of pages	5
Journal	Archives of Virology
Volume	163
Issue number	7
DOIs	https://doi.org/10.1007/s00705-018-3788-8
Publication status	Published - Jul 1 2018

ASJC Scopus subject areas

Virology

Access to Document

10.1007/s00705-018-3788-8

Fingerprint Dive into the research topics of 'Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan'. Together they form a unique fingerprint.

Cite this

Ujihara, T., Uchiyama, J., Nasukawa, T., Ando, H., Murakami, H., Ohara, N., Ogawa, M., Yamazaki, T., Daibata, M., Sakaguchi, M., & Matsuzaki, S. (2018). Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan. Archives of Virology, 163(7), 1915-1919. https://doi.org/10.1007/s00705-018-3788-8

Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan. / Ujihara, Takako; Uchiyama, Jumpei; Nasukawa, Tadahiro; Ando, Hiroki; Murakami, Hironobu; Ohara, Naoya; Ogawa, Midori; Yamazaki, Toshio; Daibata, Masanori; Sakaguchi, Masahiro; Matsuzaki, Shigenobu.

In: Archives of Virology, Vol. 163, No. 7, 01.07.2018, p. 1915-1919.

Research output: Contribution to journal › Article

Ujihara, Takako ; Uchiyama, Jumpei ; Nasukawa, Tadahiro ; Ando, Hiroki ; Murakami, Hironobu ; Ohara, Naoya ; Ogawa, Midori ; Yamazaki, Toshio ; Daibata, Masanori ; Sakaguchi, Masahiro ; Matsuzaki, Shigenobu. / Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan. In: Archives of Virology. 2018 ; Vol. 163, No. 7. pp. 1915-1919.

@article{1a96de960b5a4dcb842e0d30dd52feed,

title = "Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan",

abstract = "Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.",

author = "Takako Ujihara and Jumpei Uchiyama and Tadahiro Nasukawa and Hiroki Ando and Hironobu Murakami and Naoya Ohara and Midori Ogawa and Toshio Yamazaki and Masanori Daibata and Masahiro Sakaguchi and Shigenobu Matsuzaki",

year = "2018",

month = jul,

day = "1",

doi = "10.1007/s00705-018-3788-8",

language = "English",

volume = "163",

pages = "1915--1919",

journal = "Archives of Virology",

issn = "0304-8608",

publisher = "Springer Wien",

number = "7",

}

TY - JOUR

T1 - Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

AU - Ujihara, Takako

AU - Uchiyama, Jumpei

AU - Nasukawa, Tadahiro

AU - Ando, Hiroki

AU - Murakami, Hironobu

AU - Ohara, Naoya

AU - Ogawa, Midori

AU - Yamazaki, Toshio

AU - Daibata, Masanori

AU - Sakaguchi, Masahiro

AU - Matsuzaki, Shigenobu

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

AB - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

UR - http://www.scopus.com/inward/record.url?scp=85048470792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048470792&partnerID=8YFLogxK

U2 - 10.1007/s00705-018-3788-8

DO - 10.1007/s00705-018-3788-8

M3 - Article

C2 - 29511830

AN - SCOPUS:85048470792

VL - 163

SP - 1915

EP - 1919

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 7

ER -