Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

Takako Ujihara, Jumpei Uchiyama, Tadahiro Nasukawa, Hiroki Ando, Hironobu Murakami, Naoya Ohara, Midori Ogawa, Toshio Yamazaki, Masanori Daibata, Masahiro Sakaguchi, Shigenobu Matsuzaki

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Abstract

Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

Original languageEnglish
Pages (from-to)1915-1919
Number of pages5
JournalArchives of Virology
Volume163
Issue number7
DOIs
Publication statusPublished - Jul 1 2018

ASJC Scopus subject areas

  • Virology

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    Ujihara, T., Uchiyama, J., Nasukawa, T., Ando, H., Murakami, H., Ohara, N., Ogawa, M., Yamazaki, T., Daibata, M., Sakaguchi, M., & Matsuzaki, S. (2018). Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan. Archives of Virology, 163(7), 1915-1919. https://doi.org/10.1007/s00705-018-3788-8