Abstract
Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
| Original language | English |
|---|---|
| Pages (from-to) | 1915-1919 |
| Number of pages | 5 |
| Journal | Archives of Virology |
| Volume | 163 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2018 |
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ASJC Scopus subject areas
- Virology
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Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan. / Ujihara, Takako; Uchiyama, Jumpei; Nasukawa, Tadahiro; Ando, Hiroki; Murakami, Hironobu; Oohara, Naoya; Ogawa, Midori; Yamazaki, Toshio; Daibata, Masanori; Sakaguchi, Masahiro; Matsuzaki, Shigenobu.
In: Archives of Virology, Vol. 163, No. 7, 01.07.2018, p. 1915-1919.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan
AU - Ujihara, Takako
AU - Uchiyama, Jumpei
AU - Nasukawa, Tadahiro
AU - Ando, Hiroki
AU - Murakami, Hironobu
AU - Oohara, Naoya
AU - Ogawa, Midori
AU - Yamazaki, Toshio
AU - Daibata, Masanori
AU - Sakaguchi, Masahiro
AU - Matsuzaki, Shigenobu
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
AB - Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
UR - http://www.scopus.com/inward/record.url?scp=85048470792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048470792&partnerID=8YFLogxK
U2 - 10.1007/s00705-018-3788-8
DO - 10.1007/s00705-018-3788-8
M3 - Article
C2 - 29511830
AN - SCOPUS:85048470792
VL - 163
SP - 1915
EP - 1919
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 7
ER -